Affinity purification of plasminogen activator inhibitor I using

Details Affinity purification of plasminogen activator inhibitor I using Affinity purification of plasminogen activator inhibitor I using

1990-06-04

1991-12-17

Moskowitz, Margaret

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530413, 435 692, 435212, 435215, C07K 318, C07K 1506

Patent

active

050736264

ABSTRACT:
A method of purifying PAI-1 from a biological fluid source, e.g. conditioned media of Hep G2 or HT 1080 cells, containing PAI-1 is disclosed, which comprises subjecting the biological fluid to a modified urokinase affinity absorbent, e.g. anhydrourokinase ligand bound to a CNBr-activated agarose gel or urokinase mutated at amino acid position 356 from Ser to Gly and bound to the gel, and then eluting PAI-1 from said affinity absorbent. A method of stabilizing and/or activating PAI-1 is also disclosed, which comprises complexing PAI-1 with vitronectin.

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