Affinity matrices with enhanced visibility for molecular...

Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...

Reexamination Certificate

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C210S656000, C210S198200, C210S502100, C502S402000, C502S404000

Reexamination Certificate

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10956825

ABSTRACT:
An affinity matrix for use in affinity based molecular pull down and immunoprecipitation procedures. The affinity matrix comprises a polymeric support, a dye attached to a fraction of the polymeric support to enable optical detection of the polymeric support, and an affinity ligand other than the dye attached to a fraction of the polymeric support for the capture of a molecule.Also provided is a method for the isolation of a biomolecule from an aqueous solution. The method comprises combining the aqueous solution with an affinity matrix comprising a polymeric support and separating the affinity matrix from the aqueous solution. A dye is attached to a fraction of the polymeric support which enables optical detection and monitoring of the affinity matrix and, accordingly, reduces the likelihood of the loss of affinity matrix during the separation step. In addition, an affinity ligand other than the dye is also attached to a fraction of the polymeric support for the capture of the biomolecule.

REFERENCES:
patent: 3985620 (1976-10-01), Karges
patent: 4016133 (1977-04-01), Hyosu et al.
patent: 4373932 (1983-02-01), Gribnau et al.
patent: 4431544 (1984-02-01), Atkinson et al.
patent: 4546161 (1985-10-01), Harvey et al.
patent: 4605630 (1986-08-01), Kung et al.
patent: 4707523 (1987-11-01), Chang et al.
patent: 4716219 (1987-12-01), Eggimann et al.
patent: 4880915 (1989-11-01), Kajihara et al.
patent: 5030697 (1991-07-01), Hugl et al.
patent: 5135627 (1992-08-01), Soane
patent: 5232830 (1993-08-01), Van Ness
patent: 5395688 (1995-03-01), Wang et al.
patent: 5431807 (1995-07-01), Frechet et al.
patent: 5439591 (1995-08-01), Pliura et al.
patent: 5522994 (1996-06-01), Frechet et al.
patent: 5587323 (1996-12-01), Lewis et al.
patent: 5597485 (1997-01-01), Mazza et al.
patent: 5622872 (1997-04-01), Ribi
patent: 5643721 (1997-07-01), Spring et al.
patent: 5728457 (1998-03-01), Frechet et al.
patent: 5876597 (1999-03-01), Mazza et al.
patent: 5914241 (1999-06-01), Valkirs
patent: 6017496 (2000-01-01), Nova et al.
patent: 6242185 (2001-06-01), Kaser et al.
patent: 6428704 (2002-08-01), Setoguchi et al.
patent: 0 407 188 (1991-01-01), None
patent: 0 455 735 (1991-11-01), None
patent: 0 621 074 (1994-10-01), None
patent: WO 84/02193 (1984-06-01), None
patent: WO 90/04609 (1990-05-01), None
patent: WO 94/15951 (1994-07-01), None
patent: WO 97/09068 (1997-03-01), None
patent: WO 97/12994 (1997-04-01), None
patent: WO 98/08603 (1998-03-01), None
patent: WO 99/10370 (1999-03-01), None
patent: WO 00/07024 (2000-02-01), None
patent: WO 00/13706 (2000-03-01), None
J. Birch et al. “Affinity Chromatography: Its Role in Industry” Biotechnology Applications and Research, Technomic Publishing Company, Inc. Lancaster, PA (1985) pp. 594-606.
C. Lowe et al. “Affinity Chromatography on Immobilized Dyes” Methods in Enzymology, vol. 104, (1984) Chapter 4, pp. 97-112.
D. Stewart et al. “Affinity Chromatography on Triazine Dyes Immobilized on Novel Perfluorocarbon Supports” Journal of Biotechnology, vol. 11 (1989) pp. 253-266.
G. Kopperschlager et al. “Affinity Partitioning: A New Approach for Studying Dye-Protein Interactions” Journal of Chromatography, vol. 376 (1986) pp. 141-148.
R. Delage-Mourroux et al. “Analysis of Estrogen Receptor Interaction with a Repressor of Estrogen Receptor Activity (REA) and the Regulation of Estrogen Receptor Transcriptional Activity by REA” The Journal of Biological Chemistry, vol. 275, No. 46 (2000) pp. 35848-35856.
E. Vignal et al. “Characterization of TCL, a New GTPase of the Rho Family Related to TC10 and Cdc42” The Journal of Biological Chemistry, vol. 275, No. 46 (2000) pp. 36457-36464.
K. Giuliano “Chromatography of Proteins on Columns of Polyvinylpolypyrrolidone Using Absorbed Textile Dyes as Affinity Ligands” Analytical Biochemistry, vol. 200 (1992) pp. 370-375.
C. Jones et al. “Current Trends in Molecular Recognition and Bioseparation” Journal of Chromatography A. vol. 707 (1995) pp. 3-22.
A. Berg et al. “Dye-Ligand Centrifugal Affinity Chromatography” Bioseparation, vol. 1, No. 1 (1990) pp. 23-31.
P. Jungblut et al. “Dye Ligand Chromatography and Two-Dimensional Electrophoresis of Complex Protein Extracts from Mouse Tissue” Journal of Chromatography, vol. 482, No. 1 (1989) pp. 125-132.
E. Harlow et al. “Immunoprecipitation” Antibodies: A Laboratory Manual, Cold Spring Harbor Press, NY (1988) Chapter 7, pp. 223-255.
C. Bruton et al. “Large-Scale Purification of Enzymes” Phil. Trans. R. Soc. Lond. B, vol. 300 (1983) pp. 249-261.
J. Noriega et al. “Modeling Column Regeneration Effects on Dye-Ligand Affinity Chromatography” Biotechnology Progress, vol. 13 (1997) pp. 296-300.
D. Nash et al. “Modification of Polystyrenic Matrices for the Purification of Proteins II. Effect of the Degree of Glutaraldehyde-poly(vinyl alcohol) Crosslinking on Various Dye Ligand Chromatography Systems” Journal of Chromatography A, vol. 776 (1997) pp. 55-63.
D. Nash et al. “Modification of Polystyrenic Matrices for the Purification of Proteins III. Effects of Poly(vinyl alcohol) modification on the Characteristics of Protein Adsorption on Conventional and Perfusion Polystyrenic Matrices” Journal of Chromatography A, vol. 776 (1997) pp. 55-73.
A. Kumar et al. “Polymer Displacement/Shielding in Protein Chromatography” Journal of Chromatography B, vol. 741 (2000) pp. 103-113.
E. Phizicky et al. “Protein-Protein Interactions: Methods for Detection and Analysis” Microbiological Reviews, vol. 59, No. 1 (1995) pp. 94-123.
P. Boyer et al. “Protein Purification by Dye-Ligand Chromatography” Advances in Biochemical Engineering/Biotechnology, vol. 49, pp. 1-44.
P. Dean et al. “Protein Purification Using Immobilized Triazine Dyes” Journal of Chromatography, vol. 165 (1979) pp. 301-319.
G. Gunzer et al. “Purification of α1-Proteinase Inhibitor by Triazine Dye Affinity Chromatography, Ion-Exchange Chromatography and Gel Filtration on Fractogel TSK” Journal of Chromatography, vol. 296 (1984) pp. 221-229.
H. Mottl “Rapid Screening of a Large Number of Immobilized Textile Dyes for the Purification of Proteins: Use of Penicillin-Binding Protein 4 ofEscherichia colias a Model Enzyme” Protein Expression and Purification, vol. 3 (1992) pp. 403-409.
M'H. Hasnaoui et al. “Screening of a Large Number of Dyes for the Separation of Human Immunoglobulin G2 from the Other Immunoglobulin G Subclasses Immunoglobulin G2 Enrichment on Immobilized Procion Yellow HE-4R” Journal of Chromatography A, vol. 766 (1997) pp. 49-60.
R. Scopes “Strategies for Enzyme Isolation Using Dye-Ligand and Related Adsorbents” Journal of Chromatography, vol. 376 (1986) pp. 131-140.
R. Nehring et al. “The Metabotropic GABA8Receptor Directly Interacts with the Activating Transcription Factor 4” The Journal of Biological Chemistry, vol. 275, No. 45 (2000) pp. 35185-35191.
C. Stead “The Use of Dyes in Protein Purification” Bioseparation, vol. 2, No. 3 (1991) pp. 129-136.
J. Scoble et al. “Well Defined Dye Absorbents for Protein Purification” Journal of Molecular Recognition, vol. 9 (1996) pp. 728-732.
Snyder “Characteristics and Use of Different Column Packing” Introduction to Modern Liquid Chromatography (1979) pp. 181-183, John Wiley & Sons, New York.
Mikes, Laboratory Handbook of Chromatographic and Allied Methods (1979) pp. 347-351 and 387-389, John Wiley and Sons, New York.

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