Affinity immunoassay system

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals

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436528, 436529, 436531, 436804, 436822, 436823, 435 4, 435 7, G01N 3354, G01N 3356

Patent

active

045045852

ABSTRACT:
An affinity immunoassay system in which a solid phase nonimmunological, group-specific ligand is used to insolubilize the analyte of interest either simultaneously, before or after binding all of the analyte with a labelled monospecific antibody and the concentration of the analyte is then determined by measuring the label activity present in the solid phase in relation to a single point calibrator solution containing a known amount of the analyte substance.

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Engvall, E. et al., Int. Journal of Cancer, vol. 20, pp. 1-5 (1977).
Engvall, E. et al., J. of Experimental Medicine, pp. 1584-1595 (1978).
Engvall, E. et al., Methods in Enzymology, vol. 20, pp. 419-439 (1980).

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