Affinity column and process for detection of low molecular weigh

Details Affinity column and process for detection of low molecular weigh Affinity column and process for detection of low molecular weigh

1985-02-28

1989-04-04

Warden, Robert J.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436529, 436548, 436815, 436822, 436824, 436825, G01N 33543, G01N 33548, G01N 33577

Patent

active

048186872

ABSTRACT:
An affinity matrix and a method for the detection of low molecular weight compositions such as aflatoxins are provided utilizing specific monoclonal IgM antibody having an affinity constant not less than about 1.times.10.sup.9 liters per mole. Methods for the preparation and use of such affinity matrices are also given. The detection is rapid, accurate, reproducible, and allows for quantitative recovery of the composition of interest.

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J. D. Groopman et al., Proc. Natl. Acad. Sci., USA, 81, 7728-7731, 1984.
A. Haugen et al., Proc. Natl. Acad. Sci., USA, 78, 4124-4127, 1981.
S. Wu et al., Chem. Abs., 100(7): 46441f, 1984.
Harris and Sun, Carcinogenesis, 5, No. 6, pp. 697-701 (1984).
Hertzog et al., Carcinogenesis, 3, No. 7, pp. 825-828 (1982).
Groopman et al., Cancer Research, 42, pp. 3120-3124 (1982).
Wu et al., Chin. J. Oncol., 6 (1984).

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