Affinity biotinylation

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 74, 435 77, 435 772, 435 78, 435 792, 435 793, 435 794, 435 795, 435969, 435970, 436501, 436526, 436529, 436530, 436531, 436534, 436544, 436827, 530413, G01N 3353, G01N 33573, G01N 33531, A23J 100

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active

061501239

ABSTRACT:
The present invention relates to a method for biotinylating a desired subset of a larger population of proteins, wherein the subset of proteins is first selectively bound to a solid phase, thereby separating it from undesired proteins of the population, and then, still bound to solid phase, it is biotinylated. Unreacted biotin may then be washed from the solid phase, and the biotinylated protein may be uncoupled from the solid phase.

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