Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1992-06-19
1993-08-31
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4351721, 935 52, 935 53, 935 85, C12N 1587, C12N 1589, C12N 1590
Patent
active
052408426
ABSTRACT:
The present invention uses aerosol beam technology to accelerate either wet or dry aerosol particles to speeds enabling the particles to penetrate living cells. Aerosol particles suspended in an inert gas are accelerated to a very high velocity during the jet expansion of the gas as it passes from a region of higher gas pressure to a region of lower gas pressure through a small orifice. The accelerated particles are positioned to impact a preferred target, for example, a plant or animal cell or bacterial culture. When the droplets include DNA or other macromolecules, the macromolecules are introduced into the cells. The particles are constructed as droplets of a sufficiently small size so that the cells survive the penetration. Once introduced into the target cell the macromolecules can elicit biological effects. Because the method of introduction is a physical one, the biological barriers that restrict the application of other DNA transfer methods to a few plant species and a few cell types are not present. In addition, the method and apparatus of the present invention permit the treatment of a large number of cells in the course of any single treatment. Thus, the inventive method and apparatus should be applicable to a wide range of plant species and cell types that have proved in the past to be quite impervious to standard methods of genetic engineering.
REFERENCES:
patent: 4945050 (1990-07-01), Sanford et al.
patent: 5141131 (1992-08-01), Miller et al.
Wastie 1984 Plant Pathology 33: 61-63.
Moynahan et al 1965 British Medical Journal 3: 1541-1543.
Lachapelle et al, 1982 Ann Dermatol. Venereol. (Paris) 109: 939-946.
Sanford, John C. et al., Delivery of substances into cells and tissues using a particle bombardment process. Particulate Science and Technology 5:27-37, 1987.
Johnston, Stephen A. et al., Mitochondrial Transformation in Yeast by Bombardment with Microprojectiles. Science, vol. 240 pp. 1538-1540.
Sanford, John C., The Biolistic process. Tibtech--Dec. 1988 vol. 6, pp. 299-302.
Sanford, John, The Biolistic Process. Amer. Soc. Plant Physiologists Aug. 1989, pp. 2.
Christou, Paul et al., Soybean transformation using electric discharge particle acceleration. Amer. Soc. Plant Physiologists Aug. 1989 pp. 2.
Boynton, John E. et al., Chloroplast Transformation in Chlamydomonas with High Velocity Microprojectiles.Science, vol. 240 pp. 1534-1537.
Klein, T. N., et al., High-velocity microprojectiles for delivering nucleic acids into living cells. Nature vol. 327 May 1987, pp. 70-73.
Wang, Yi-Chang et al., Transient expresson of foreign genes in rice, wheat and soybean cells following particle bombardment. Plant Molecular Biology 11:433-439.
Klein, Theodore M. et al., Stable genetic transformation of intact Nicotiana cells by the particle bombardment process. Proc. Natl. Acad. Sci. USA vol. 85, pp. 8502-8505, Nov. 1988.
Christou, Paul et al., Stable Transformation of Soybean Callus by DNA-Coated Gold Particles. Plant Physiol, 1988, 87, 671-674.
Bryant, John A. Putting genes into plants. Plants today Jan.-Feb. 1988/23.
Lee, Bruce, Cereal transformation. Plants Today Jan.-Feb. 1989/9.
Chupeau, Marie-Christine, et al., Transgenic Plants of Lettuce (Lactuca sativa) Obtained through Electroporation of Protoplasts. Bio/Technology vol. 7 May 1989, pp. 503-508.
Chassy, Bruce M., et al., Tansformation of bacteria by electroporation. Tibtech--Dec. 1988 vol. 6, pp. 303-309.
Bio Rad. Gene Pulser Electroporation Apparatus, Bulletin 1272.
Dahneke, B. E., et al., Properties of Continuum Source Particle Beams. I. Calculation Methods and Results. J. Aerosol Sci. vol 10, pp. 257-274.
Dahneke, Barton, et al., Prog. Astronautics & Aeronautics 74:504-514 (1981), pp. 504-514.
Israel, Gerhard, et al., High-Speed Beams of Small Particles. Journal of Colloid and Interface Science 24, 330-337, 1967.
Mora de la, Fernandez J., Surface impact of seeded jets at relatively large background densities. Yale University 1984.
Dahneke, Barton, et al., An Aerosol Beam Spectrometer. Aerosol Science, 1972 vol. 3, pp. 345-349.
Mayer, Erwin, New method for vitrifying water and other liquids by rapid cooling of their aerosols. American Inst. of Physics. Jul. 1985, pp. 663-667.
Biotechnology Research And Development Corporation
Moody P.
Weimar Elizabeth C.
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