Aerobic succinate production in bacteria

Details Aerobic succinate production in bacteria Aerobic succinate production in bacteria

2011-05-03

2011-05-03

Kelly, Robert M (Department: 1633)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S144000, C435S145000, C435S252100, C435S252300, C435S440000, C435S471000, C435S488000

Reexamination Certificate

active

07935511

ABSTRACT:
Methods of increasing yields of succinate using aerobic culture methods and a multi-mutantE. colistrain are provided. Also provided is a mutant strain ofE. colithat produces high amounts of succinic acid.

REFERENCES:
patent: 5393615 (1995-02-01), Corey et al.
patent: 5520786 (1996-05-01), Bloczynski et al.
patent: 6455284 (2002-09-01), Gokarn et al.
patent: 6743610 (2004-06-01), Donnelly et al.
patent: 7223567 (2007-05-01), Ka-Yiu et al.
patent: 7244610 (2007-07-01), San et al.
patent: 7262046 (2007-08-01), Ka-Yiu et al.
patent: 2003/0087381 (2003-05-01), Gokarn et al.
patent: 2005/0042736 (2005-02-01), San et al.
patent: 2006/0073577 (2006-04-01), Ka-Yiu et al.
patent: 2006/0141594 (2006-06-01), San et al.
Abdel-Hamid, A.M., et al., Pyruvate oxidase contributes to the aerobic growth efficiency ofEscherichia coli. Microbiol. 2001, 147:1483-1498.
Chou, C., et al.; Effect of Modulated Glucose Uptake on High-Level Recombinant Protein Production in a DenseE. coliCulture; Biotechnol. Prog., vol. 10, pp. 644-647, 1994.
Dittrich, C. R.; Vadali, R. V.; Bennett, G. N.; San, K.-Y. Redistribution of metabolic fluxes in the central aerobic metabolic pathway ofE. colimutant strains with deletion of the ackA-pta and poxB pathways for the production of isoamyl acetate. 2004.
Gokarn, R. R.; Eiteman, M. A.; Altman, E. Expression of pyruvate carboxylase enhances succinate production inEscherichia coliwithout affecting glucose uptake rate. Biotech. Let. 1998, 20, 795-798.
Gokarn, R. R.; Eiteman, M. A.; Altman, E. Metabolic analysis ofEscherichia coliin the presence and absense of the carboxylating enzymes phosphoenolpyruvate carboxylase and pyruvate carboxylase. Appl Environ Microbiol. 2000, 666, 1844-1850.
Hahm, D. H.; Pan, J. G.; Rhee, J. S. Characterization and evaluation of a pta (phosphotransacetylase) negative mutant ofEscherichia coliHZB101 as a production host of foreign lipase. Appl Microbiol Biotechnol. 1994, 42, 100-107.
Helling et al., J. Bact. 1971, 105:1224-1226.
Holms, W. H. The central metabolic pathways inEscherichia coli: relationship between flux and control at a branchpoint, efficiency of conversion to biomass, and excretion of acetate. Curr Top Cell Regul. 1986, 28, 69-105.
Hong, et al., Biotechnol. Bioeng. 2001, 74:89-95.
Kornberg, H. L. The role and control of the glyoxylate cycle inEscherichia coli. Biochem. J. 1966, 99, 1-11.
Kubo et al., J. Biosc. Bioeng. 2000, 6:619-624.
Lin H, Bennett GN, San KY. Effect of carbon sources differing in oxidation state and transport route on succinate production in metabolically engineeredEscherichia coli. J Ind Microbiol Biotechnol. Mar. 16, 2005; [Epub ahead of print].
Lin H, Bennett GN, San KY. Genetic reconstruction of the aerobic central metabolism inEscherichia colifor the absolute aerobic production of succinate. Biotechnol Bioeng. Jan. 20, 2005;89(2):148-56.
Lin H, San KY, Bennett GN. Effect ofSorghum vulgarephosphoenolpyruvate carboxylase andLactococcus lactispyruvate carboxylase coexpression on succinate production in mutant strains ofEscherichia coli.Appl Microbiol Biotechnol. Nov. 24, 2004; [Epub ahead of print].
Lin H, Vadali RV, Bennett GN, San KY. Increasing the acetyl-CoA pool in the presence of overexpressed phosphoenolpyruvate carboxylase or pyruvate carboxylase enhances succinate production inEscherichia coli. Biotechnol Prog. Sep.-Oct. 2004;20(5):1599-604.
Luli, G. W.; Strohl, W. R. Comparison of growth, acetate production, and acetate inhibition ofEscherichia colistrains in batch and fed-batch fermentations. Applied and Environmental Microbiology. 1990, 56, 1004-1011.
Lin H. et al., Metabolic engineering of aerobic succinate production systems inEscherichia colito improve process productivity and achieve the maximum theoretical succinate yield. Metab Eng. Mar. 2005;7(2):116-27.
Phillips, G. J.; Park, S. K.; Huber, D. High copy number plasmids compatible with commonly used cloning vectors. Biotechniques. 2000, 28, 400-408.
Sunnarborg et al., J. Bacteriol. 1990, 172:2642-2649.
Vemuri, et al., J. Ind. Microbiol. Biotechnol. 2002, 28:325-332.
Vemuri, G. N.; Eiteman, M. A.; Altman, E. Effect of growth mode and pyruvate carboxylase on succinic acid production by metabolically engineered strains ofEscherichia coli. Appl Environ Microbiol. 2002, 68, 1715-1727.
Yang, Y.T., et al., The effects of feed and intracellular pyruvate levels on the redistribution of metabolic fluxes inEscherichia coli. Metabolic Engineering. 2001, 3:115-123.
Yang YT, Peredelchuk M, Bennett GN, San KY. Effect of variation ofKlebsiella pneumoniaeacetolactate synthase expression on metabolic flux redistribution inEscherichia coli. Biotechnol Bioeng. Jul. 20, 2000;69(2):150-9.
Yang YT, Aristidou AA, San KY, Bennett GN. Metabolic flux analysis ofEscherichia colideficient in the acetate production pathway and expressing theBacillus subtilisacetolactate synthase. Metab Eng. Jan. 1999;1(1):26-34.
Brown, T.D.K., et al., The enzymic interconversion of acetate and acetyl-coenzyme A inEscherichia coli. Journal of General Microbiology 102:327-336 (1977).

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