Aequorin as a growth marker in yeast

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S029000, C435S254200, C435S483000

Reexamination Certificate

active

07622275

ABSTRACT:
A yeast cell containing constitutively expressed aequorin and methods of using the cells in growth and toxicity assays are disclosed.

REFERENCES:
patent: 5876951 (1999-03-01), Fowlkes et al.
patent: 2002/0025514 (2002-02-01), Jaworski et al.
Nakajima-Shimada et al. Galactose-dependent expression of the recombinant Ca2(+)-binding photoprotein aequorin in yeast. Biochem Biophys Res Commun. Jan. 15, 1991;174(1):115-22, Jan. 1991.
Inouye et al. Expression of apoaequorin complementary DNA inEscherichia coli. Biochemistry, vol. 25, pp. 8425-8429, 1986.
Paz et al. Monitoring dynamics of gene expression in yeast during stationary phase. Gene, vol. 236, No. 1, pp. 33-42, Aug. 1999.
Alber Tom et al., Nucleotide Sequence Of The Triose Phosphate Isomerase Gene OfSaccharomyces cervisiae, Journal Of Molecular And Applied Genetics, 1, (1982), pp. 419-434.
Brini Marisa et al., Targeted Recombinant Aequorins: Tools For Monitoring [Ca2+] In The Various Compartments Of A Living Cell, Microscopy Research And Technique (1999) 46 pp. 380-389.
Iida Hidetoshi et al., Essential Role For Induced Ca2+ Influx Followed By [Ca2+] Rise In Maintaining Viability Of Yeast Cells Late In the Mating Pheromone Response Pathway, The Journal Of Biological Chemistry (1990) 265 pp. 13391-13399.
Inouye Satoshi et al., Overexpression And Purification Of The Recombinant Ca2+ Binding Protein, Apoaequorin, J. Biochem (1989) 105 pp. 473-477.
Ito Hisao et al., Transformation Of Intact Yeast Cells Treated With Alkali Cations, Journal Of Bacteriology (1983) pp. 163-168.
Johnson Frank H. et al., Introduction To The Bioluminescence Of Medusae, With Special Reference To The Photoprotein Aequorin, Methods In Enzymology (1978) LVII pp. 1-653.
King Klim et al., Control Of Yeast Mating Signal Transduction By A Mammalian B2-Adrenergic Receptor And Gs Subunit, Erratum Appears In Science (1990) pp. 121-123.
Miller Andrew L. et al., Imaging [Ca2+]i With Aequorin Using A Photon Imaging Detector, Methods In Cell Biology (1994) 40 pp. 305-338.
Nakajima-Shimada Junko et al., Ca2+ Signal is Generated Only Once In the Mating Pheromone Response Pathway inSaccharomyces cerevisiae, Cell Structure and Functin 25, (2000) pp. 125-131.
Nakajima-Shimada Junko et al., Monitoring Of Intracellular Calcium InSaccharomyces cerevisiaeWith An Apoaequrin cDNA Expression System, Proc. Natl. Acad. Sci. USA, vol. 88, Cell Biology, Aug. 1991, pp. 6878-6882.
Ohimiya Yoshihiro et al., Shining The Light: The Mechanism Of The Bioluminescence Reaction Of Calcium-Binding Photoproteins, Chemistry & Biology (1996) 3 pp. 337-347.
Parnot Charles et al., Systematic Identification Of Mutations That Constitutively Activate The Angiotensin II Type 1A Receptor By Screening A Randomly Mutated cDNA Library With An Original Pharmacological Bioassay, PNAS, vol. 97, No. 13, Jun. 20, 2000, pp. 7615-7620.
Rizzuto Rosario et al., Rapid Changes Of Mitochondrial Ca2+ Revealed By Specifically Targeted Recombinant Aequorin, Nature (1992) 358 pp. 325-327.
Sheu Yeong-An et al., Measurement Of Intracellular Calcium Using Bioluminescent Aequorin Expressed In Human Cells, Analytical Biochemistry (1993) 209 pp. 343-347.
Shimomura Osamu et al., Peroxidized Coelenterazine, The Active Group In The Photoprotein Aequorin, Proc. Nat'l. Acad. Sci. USA (1978) 75 pp. 2611-2615.
Stables Jenny et al., Recombinant Aequorin As Reporter Of Changes In Intracellular Calcium In Mammalian Cells, Methods In Enzymology (2000) 327 pp. 456-471.
Thomas Andrew P. et al., The Use Of Fluorescent Indicators For Measurements Of Cytosolic-Free Calcium Concentration In Cell Populations And Single Cells, A Pratical Approach (McCormack JG and Cobbold PH eds) (1991) pp. 1-54.
Iida et al., Durable Synthesis of High Molecular Weight Heat Shock Proteins in Go Cells of the Yeast and Other Eucaryotes, J. of Cell Biology, vol. 99, Jul. 1984, pp. 199-207.
Tanahashi et al., Photoprotein aequorin: use as a reporter enzyme in studying gene expression in mammalian cells, Gene, vol. 96, 1990, pp. 249-255.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Aequorin as a growth marker in yeast does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Aequorin as a growth marker in yeast, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Aequorin as a growth marker in yeast will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4054566

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.