Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-11-04
1997-05-06
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 435348, C12P 2106, C12N 506, C12N 1586
Patent
active
056270488
ABSTRACT:
The present invention is directed to use of Aedes aegypti densonucleosis virus (Aedes DNV or AeDNV) as a heterologous gene expression system. The invention described herein provides promoters from the Aedes aegypti genome such as the p.05, p7 and p61 promoters to generate expression vectors with cloning sites for insertion of heterologous genes. The invention further provides expression vectors comprising an AeDNV promoter operably linked to a heterologous gene and insect host cells comprising the vectors of the invention. Methods of producing recombinant proteins utilizing the vectors of the invention are also provided. The present invention further provides a kit containing the vectors of the invention for production of a recombinant protein.
REFERENCES:
Afanasiev et al. (Nov. 17,1994) "Densovirus of Aedes aegypti as an expression vector in mosquito cells" Experimental Parasitology 79:322-339.
Boublik et al. (1994) "Complete nucleotide sequence and genomic organization of the Aedes albopictus parvovirus (AaPv) pathogenic for Aedes aegypti larvae" Virology 200(2):752-763.
Giraud et al., Virology (Jan., 1992), vol. 186: pp. 207-218.
Afanasiev et al., Virology (1991), vol. 185: pp. 323-336.
Lebedinets et al., Mikrobiologicheskii Zhurnal (1978),vol. 40(3): pp. 352-356.
Igarashi, J. of General Virology (Sep., 1978), vol. 40: pp. 531-544.
Afanasiev Boris N.
Beaty Barry
Carlson Jonathan O.
Higgins David R.
Thibault Kelly J.
Fleisher Mindy
McKelvey Terry A.
Research Corporation Technologies Inc.
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