Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-07
1998-12-15
Elliott, George C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 435348, C12P 2106, C12N 506, C12N 1586
Patent
active
058495231
ABSTRACT:
The present invention is directed to use of Aedes aegypti densonucleosis virus (Aedes DNV or AeDNV) as a heterologous gene expression system. The invention described herein provides promoters from the Aedes aegypti genome such as the p.05, p7 and p61 promoters to generate expression vectors with cloning sites for insertion of heterologous genes. The invention further provides expression vectors comprising an AeDNV promoter operably linked to a heterologous gene and insect host cells comprising the vectors of the invention. Methods of producing recombinant proteins utilizing the vectors of the invention are also provided. The present invention further provides a kit containing the vectors of the invention for production of a recombinant protein.
REFERENCES:
Afanasiev et al. (1991) "Nucleotide sequence and genomic organization of Aedes Densonucleosis virus" Virology 185:323-336.
Giraud et al. (1992) "The densovirus of Junonia-coenia (JcDNV) as an insect cell expression vector" Virology 186:207-218.
Igarashi et al. (1978) "Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses" J. Gen. Virol. 40:531-544.
Lebedinets et al. (1978) "A study of the effect of the densonucleosis virus of the mosquito Aedes aegypti L. of the preadult stages of differen species of blood sucking mosquitoes" Mikrobiologicheskli Zhurnal 40:352-356.
Afanasiev et al. (Nov. 17, 1994) "Densovirus of Aedes aegypti as an expression vector in mosquito cells" Experimental Parasitology 79:322-339.
Boublik et al. (1994) "Complete nucleotide sequence and genomic organization of the Aedes albopictus parvovirus (AaPV) pathogenic for Aedes aegypti larvae" Virology 200(2):752-763.
Afanasiev Boris N.
Beaty Barry J.
Carlson Jonathan Ored
Higgins David R.
Thibault Kelly J.
Elliott George C.
McKelvey Terry A.
Research Corporation Technologies Inc.
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