Advantageous vitrifiable cryoprotectant mixtures

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including freezing; composition therefor

Reexamination Certificate

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Reexamination Certificate

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07741018

ABSTRACT:
Disclosed herein are aqueous carrier solutions for cryoprotectants that are useful for a variety of cryoprotectant solutions for use with cells, tissues, and whole organs, said aqueous carrier solutions permitting antinucleators to be fully effective in vitrification solutions, thereby allowing vitrification solutions to attain extreme effectiveness, and compatible vitrification solution compositions for use with these carrier solutions. The carrier solutions comprise lactose, mannitol or trehalose, and glucose in combination with other beneficial ingredients.

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Kheirabadi. Bijan S. and Fahy, Gregory M., Permanent Life Support by Kidneys Perfused with a Vitrifiable (7.5 Molar) Cryoprotectant Solution,Transplantation, 70: 51-57, 2000.
Khirabadi. Bijan S. and Fahy, Gregory M., Cryopreservation of the Mammalian Kidney—Transplantation of Rabbit Kidneys Perfused with EC and RPS-2 at 2-4°C1,Cryobiology, 31: 10-25, 1994.
Kirabadi, B. S., Fahy, G.M., Merei, J., and Rasch, C., Does Substitution of Mannitol for Glucose Improve Euro-Collins as a Vehicle for Cryoprotective Agents?,Cryobiology, 35: 357, 1997.
“paper submitted for publication, 2001”, Arnaud, Francoise, Khirabadi, Bijan S., Fahy, Gregory M., Normothermic blood perfusion of isolated rabbit kidneys,Transpl Int, 15: 278-289, 2002.
Fahy, Gregory M. et al., Cellular Injury Associated with Organ Cryopreservation: Chemical Toxicity and Cooling Injury,Cell Biology of Trauma, CRC Press, Boca Raton, Chapter 20: 333-356, 1995.
Rall, W. F. and Fahy, G. M., Ice-free cryopreservation of mouse embryos at—196°C by vitrification,Nature, 313: 573-575, 1985.
Williams et al., Vial breakage by frozen Mannitol solutions: Correlation with thermal characteristics and effect of stereoisomerism, additives and vial configuration. J. Parenteral Science 7 Technology, 45(2): 94-100, 1991.
Ren et al., The Crystallization kinetics and the critical cooling rates for vitrification of cryoprotective solutions. Cryogenics, 30:536-540, 1990.
Klebe et al., Identification of new cryoprotective agents for cultured mammalian cells. In Vitro, 19(3 Part 1): 167-170, 1983.
Fahy, Gregory M. and Ali, Suja E., Cryopreservation of the Mammalian Kidney,Cryobiology, 35:114-1131, 1997.
Kheirabadi, Bijan S. and Fahy, Gregory M., Permanent Life Support By Kidneys Perfused with a Vitrifiable (7.5 Molar) Cryoprotectant Solution,Transplantation, 70: 51-57, 2000.
Khirabadi, Bijan S. and Fahy, Gregory M., Cryopreservation of the Mammalian Kidney—Transplantation of Rabbit Kidneys Perfused with EC and RPS-2 at 2-4°C1Cryobiology, 31: 10-25, 1994.
Kirabadi, B. S., Fahy, G.M., Merei, J., and Rasch, C. Does Substitution of Mannitol for Glucose Improve Euro Collins as a Vehicle for Cryoprotective Agents?,Cryobiology, 35: 357, 1997.
“paper submitted for publication, 2001”, Arnaud, Francoise, Khirabadi, Bijan S., Fahy, Gregory M., Nomothermic blood perfusion of isolated rabbit kidneys,Transpl Int, 15: 278-289, 2002.
Fahey, Gregory M. et al., Cellular Injury Associated with Organ Cryoperservation: Chemical Toxicity and Cooling Injury,Cell Biology of Trauma, CRC Press, Boca Raton, Chapter 20: 333-356, 1995.
Rall, W. F. and Fahy, G. M., Ice-free cryopreservation of mouse embryos at 196°C by vitrification,Nature, 313: 573-575, 1985.

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