Advanced pathogen testing and carcass-certification methods...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

Reexamination Certificate

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C435S005000, C435S006120, C435S007100, C435S029000, C435S031000, C435S034000

Reexamination Certificate

active

07919232

ABSTRACT:
The invention provides methods for certification of carcasses, and for detecting a contaminated carcass and preventing its movement into or across a production area. The inventive methods comprise obtaining, early in the production process (pre-fabrication), a test sample from at least one test location of at least one split-portion of each carcass, wherein the test samples are obtained prior to or during chilling of the respective split portions, before entry thereof in the production chain. Composite test samples are assayed for pathogens or microbes, whereby certification is afforded, or whereby entry of the chilled split-carcass-Lot into the production area is precluded if the corresponding composite-Lot test sample is contaminated. Methods for remedial reconditioning of contaminated split-carcasses are provided, wherein essentially 100% of the carcasses are targeted to the production line. The inventive methods provide substantial public health benefit, and are efficient and economical to implement.

REFERENCES:
patent: 2002/0004091 (2002-01-01), Schaefer et al.
patent: 295349 (1988-12-01), None
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Gill et al. Microbiological sampling of carcasses by excision or swabbing. Journal of Food Protection 63(2):167-173, Feb. 2000.
Korsak et al. An efficient sampling technique used to detect four foodborne pathogens on pork and beef carcasses in nine Belgian abattoirs. Journal of Food Protection 61(5):535-541 (1998).
Kilsby, D.C. Sampling Schemes and Limits. Meat Microbiology, Chapter 10, pp. 387-421, M.H. Brown (editor), Applied Science Publishers (1982).
Siragusa et al. Monitoring the microbial contamination of beef carcass tissue with a rapid chromogenicLimulusamoebocyte lysate endpoint assay. Letters in Applied Microbiology 31:178-183 (2000).
Patil, G.P. Editorial: Composite sampling. Environmental and Ecological Statistics 2:169-179 (1995).
Elder, R.O. et al., “Correlation of enterohemorrhagicEscherichia coliO157 prevalence in feces, hides and carcasses of beef cattle during processing,” Proc. Natl. Acad. Sci. 97:299-3003, 2000.

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