Adsorption-desorption purification of glucose isomerase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Isomerase

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435815, C12N 992

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active

046109650

ABSTRACT:
The present invention relates to a process for the production of a purified glucose isomerase which comprises contacting an impure extract containing glucose isomerase and soluble impurities with a weakly basic ion exchange material known to adsorb glucose isomerase; adding a first salt solution of a concentration which removes unadsorbed and weakly adsorbed impurities, but not glucose isomerase; and adding a second, buffered salt solution which elutes the adsorbed glucose isomerase.

REFERENCES:
patent: 4256838 (1981-03-01), Jackson et al.
patent: 4263400 (1981-04-01), Ushiro

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