Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1996-04-05
1998-03-31
Degen, Nancy
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530382, 530412, 536 56, 536122, C07K 116, C07K 14745, C08B 514
Patent
active
057340250
ABSTRACT:
This invention provides a selective cellular fibronectin (cFN) adsorbent utilizing a nonwoven cellulose sulfate fabric and a method for fractional purification of FN which-comprises contacting an FN matter containing both plasma fibronectin (pFN) and cellular fibronectin (cFN) with the nonwoven cellulose sulfate fabric to separate pFN and cFN from each other. By the fractional purification method of the invention, cFN and pFN can be fractionated in an expedient manner and with high efficiency and both pFN and cFN can be recovered in high purity and good yield.
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patent: 3804092 (1974-04-01), Tunc
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patent: 4744899 (1988-05-01), Tani et al.
patent: 5324823 (1994-06-01), Asakawa et al.
patent: 5561114 (1996-10-01), Komai et al.
The Jounral of Biological Chemistry, vol. 260, No. 8, Apr. 25, 1985, pp. 5105-5114, "Differences in Domain Structure between Human Fibronectins Isolated from Plasma and from Culture Supernatants of Normal and Transformed Fibroblasts", Kiyotoshi Sekiguchi et al.
Inoue Fujio
Kamogawa Hiroshi
Komai Takashi
Miyashita Keiichi
Sakashita Eiji
Degen Nancy
Otsuka Pharnaceutical Factory, Inc.
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