Adrenomedullin

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 25 or more amino acid residues in defined sequence

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530399, 530402, A61K 5100, C07K 1400

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active

056398557

ABSTRACT:
According to the present invention, adrenomedullin which is a novel peptide having a hypotensive effect; proadrenomedullin N-terminal 20 peptide (proAM-N20) corresponding to an amino acid sequence of an N-terminus of proadrenomedullin, having a catecholamine secretion inhibitory effect; proadrenomedullin N-terminal 10-20 peptide (proAM-N(10-20)) having a Na channel inhibitory effect, and a gene encoding these peptides are provided. In addition, according to the present invention, these peptides in a sample containing adrenomedullin or proAM-N20 in an unknown amount can be quantified by using an antibody against adrenomedullin, proAM-N20, or its fragment.

REFERENCES:
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Mains et al. (1993). Trends in Neurosciences vol. 6: pp. 229-235.
Kitamura et al. (1993) Biochem & Biophys. Res. Comm. vol. 194 pp. 720-725.
G. Cooper et al., "Purification and Characterization of a Peptide from Amyloid-rich Pancreases of Type 2 Diabetic Patients", Proc. Natl. Acad. Sci. USA, 84, pp. 8628-8632 (1987).
K. Kangawa & H. Matsuo, "Purification and Complete Amino Acid Sequence of .alpha.-Human Atrial Natriuretic Polypeptide (.alpha.-hANP)", Biochem. Biophys. Res. Commun., 118, pp. 131-139 (1984).
T. Kita et al., "Effects of Brain Natriuretic Peptide-45, a Circulating Form of Rat Brain Natriuretic Peptide. in Spontaneously Hypertensive Rats", Eur. J. Pharmacol., 202, pp. 73-79 (1991).
K. Kitamura et al., "Isolation and Characterization of Peptides which Act on Rat Platelets, from a Phenochromocytoma", Biochem. Biophys. Res. Commun., 185, pp. 134-141 (1992).
K. Mizuno, "Biochemical Studies of Enzymes Involved in Processing of Biologically Active Peptide", Seikagaku, 61, pp. 1435-1461 (1989).
H. Morris et al, "Isolation and Characterization of Human Calcitonin Gene-related Peptide", Nature, 308, pp. 746-748 (1984).
P. Steenbergh et al, "Second Human Calcitonin/CGRP Gene", FEBS Lett., 2488, pp. 403-407 (1985).

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