Adipocyte-specific DNA sequences and use thereof in the producti

Details Adipocyte-specific DNA sequences and use thereof in the producti Adipocyte-specific DNA sequences and use thereof in the producti

1991-10-07

1995-12-19

Wax, Robert A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 231, 4353201, 935 36, C12N 1511, C07H 2104

Patent

active

054769264

ABSTRACT:
Regulatory DNA sequences are provided, which are obtained from the 5' flanking region of genes which are expressed primarily in differentiated adipose tissue. These DNA sequences are largely responsible for driving the expression of endogenous genes specifically in adipose tissue in vivo. The DNA sequences can be located in a region 5' of the gene, distinct from promoter sequences which provide a site for the initiation of transcription into DNA, or can be located within the region of the promoter itself.
When operatively linked to a gene encoding a recombinant protein capable of exerting an effect on the metabolism of adipocytes, the DNA sequences of the invention can be used to produce transgenic animals which exhibit altered fat tissue metabolism. Depending upon the nature of the gene introduced in the animal or ancestor thereof at an embryonic stage, the transgenic animals are leaner or more obese than non-transgenic animals of the same species.

REFERENCES:
patent: 4736866 (1988-04-01), Leder et al.
patent: 4870009 (1989-09-01), Evans et al.
patent: 4873316 (1989-10-01), Meade et al.
Platt et al. Proc. Nat. Acad. Sci., USA 86 7490-7494 (1989).
Min et al. Nucleic Acids Res. 14 8879-8892 (1986).
Phillips et al. J. Biol. Chem. 261 10821-10827.
Hresko et al. Proc. Nat. Acad. Sci., USA 85 8835-8839 (1988).
Hunt et al. Proc. Nat. Acad. Sci., USA 83 3786-3790 (1986).
Hanahan Science 246:1265-1275 (1989).
van Zonneveld et al. Proc. Nat. Acad. Sci., USA, 85 5525-5529 (1988).
Bernlohr et al., "Expression of specific mRNAs during adipose differentiation: Identification of an mRNA encoding a homologue of myelin P2 protein", Proc. Natl. Acad. Sci., U.S.A., 81:5468-5472 (Sep. 1984).
Zezulak and Green, Molecular and Cellular Biology, vol. 5, No. 2, 419-421 (Feb. 1985).
Cook et al., The Journal of Cell Biology, 100:514-520 (Feb. 1985).
Bernlohr et al., The Journal of Biological Chemistry, vol. 260, No. 9, 5563-5567 (May, 1985).
Phillips and Green, The Journal of Biological Chemistry, vol. 261, No. 3, 10821-10827 (Aug. 1986).
Distel et al., Cell, 49:835-844 (Jun., 1987).
Cook et al., Proc. Natl. Acad. Sci., USA, 85:2949-2953 (May, 1988).
Gaskins et al., J. Anim. Sci., 67:2263-2272 (1989).
Yang et al., Proc. Natl. Acad. Sci., USA, 86:3629-3633 (May, 1989).
Herrera, Molecular and Cellular Biology, vol. 9, No. 12, 5331-5339 (Dec. 1989).
Christy et al., Genes and Development, 3:1323-1335 (1989).
Wilkison et al., The Journal of Biological Chemistry, 265:477-482 (1990).
Cundiff, "The Application of Genetic Principles and Genetic Engineering to Improve Efficiency of Lean Meat Production", Reciprocal Meat Conference Proceedings, (1983).
Pursel et al., "Gene Transfer for Enhanced Growth of Livestock", Animal Growth Regulation, Chapter 16.
Fox et al., "Metatastic Hibernomas in Transgenic Mice Expressing an a-Amylase-SV40 Antigen Hybrid Gene", Science, 244:460-463 (Apr., 1989).
Hammer et al., "Use of Gene Transfer to Increase Animal Growth", Quantitative Biology, (Cold Spring Harbor Symposium) 50:379-387 (1985).
Hammer et al., "Genetic Engineering of Mammalian Embryos", J. Anim. Sci., 63:269-278 (1986).
Miller et al., "Expression of human or bovine growth hormone gene . . . ", J. of Endocrinology, 120:481-488 (1989).
Pursel et al., "Development of 1-cell and 2-cell pig ova after microinjection of genes", J. Anim. Sci., 65:402 (1987 Supp.).
Bolt et al., "Transgenic swine transmit foreign gene to progeny", J. Anim. Sci., 63:203 (1986 Supp.).
Pursel et al., "Genetic Engineering of Livestock", Science, 244:1281-1288 (Jun. 1986).
Richardson et al., "Transforming Growth Factor Type B (TGF-B) And Adipogenesis In Pigs", J. Anim. Sci., 67:2171-2180 (1989).
Sinnett-Smith and Woolliams, "Antilipogenic But Not Lipolytic Effects of Recombinant DNA-Derived Bovine Somatotropin Treatment . . . ", Int. J. Biochem., 21:535-540 (1989).
Navre and Ringold, "Differential Effects of Fibroblast Growth Factor . . . ", The Journal of Cell Biology, 109:1857-1863 (Oct. 1989).
Rexroad et al., "Gene insertion: Role and limitations of technique in farm animals as a key to growth", Invited Papers for May 3-7, 1987 Symposium at Beltsville Agricultural Research Center.
Hausman et al., "Endrocrine Regulation of Adipogenesis," Animal Growth Regulation (Campion et al., ed. 1989).
Beerman, "Status of Current Strategies for Growth Regulation", Animal Growth Regulation, (Campion et al., ed. 1989).

Affiliated with

Also associated with

Rating

Adipocyte-specific DNA sequences and use thereof in the producti does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Adipocyte-specific DNA sequences and use thereof in the producti, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Adipocyte-specific DNA sequences and use thereof in the producti will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-992703