Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Reexamination Certificate
2006-10-31
2006-10-31
Campell, Bruce R. (Department: 1654)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
C530S303000, C536S023500, C435S069400, C435S320100, C435S325000
Reexamination Certificate
active
07129211
ABSTRACT:
The present invention relates to an adipocyte Insulin adpInsl with Insulin A and B chains of SEQ ID Nos. 1 and 2, a effective method of treating type 2 diabetes in a subject using adipocyte insulin, said method comprising steps of administering the insulin to a diabetic intraperitoneally, an Insulin gene of SEQ ID No. 3, a process of isolating protein Insulin from the adipocytes of the Carp, said method comprising steps of reverse transcripting RNA of adipocytes to obtain cDNA, using oligonucleotide primers of SEQ ID Nos. 5 and 6 to identify AdpInsl gene from cDNA, and deducing amino acid sequence from cDNA to obtain protein Insulin, and an adipocyte of Catla Catla useful for producing the said Insulin.
REFERENCES:
Roy et al. A New Cell Secreting Insulin. Endocrinology, Apr. 2003, vol. 144, No. 4, pp. 1585-1593.
Makower et al. Carp Insulin: Amino Acid Sequence, Biological Activity and Structural Properties. Eur J Biochem, 1982. vol. 122, pp. 339-345.
Bhattacharya Samir
Dasgupta Subrata
Mukherjee Mohua
Roy Sib Sankar
Campell Bruce R.
Cordero Garcia Marcela M
Council of Scientific and Industrial Research
Department of Biotechnology Department of the Government of Indi
Oliff & Berridg,e PLC
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