Adenylate kinase and process for the production thereof

Details Adenylate kinase and process for the production thereof Adenylate kinase and process for the production thereof

1981-10-07

1986-04-22

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435832, 435 92, C12N 912, C12P 1932, C12R 107

Patent

active

045842728

ABSTRACT:
A heat-resistant adenylate kinase is described whose activity after an incubation in a buffer solution at about 50.degree. C. for about 15 minutes is at least about 80% of the original activity prior to the incubation. This adenylate kinase can be obtained by cultivating a bacterium belonging to the genus Bacillus and collecting adenylate kinase from the resulting culture broth. This heat-resistant enzyme is very stable against heat and, therefore, after isolation, it can be stored for a long period of time compared with the conventional adenylate kinase.

REFERENCES:
Konstantinova, Computer abstract of English Translation from Doklady Akademii Nauk SSSR Ser Biokhim, vol. 203 (1-6), No. 5 pp. 1204-1206 (pp. 121-122 of translation) 1972.
Lauwers et al, Chemical Abstracts 96: 2766k, 1982, abstract of Arch. Microbiol 1981, 130(2), 159-164.
Methods in Enzymology vol. 44, pp. 887-897.

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