Adenovirus E1-complementing cell lines

Details Adenovirus E1-complementing cell lines Adenovirus E1-complementing cell lines

1996-04-26

1999-04-06

Railey, II, Johnny F.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435371, C12N 510, C12N 1586, C12N 1587

Patent

active

058916903

ABSTRACT:
The present invention relates to adenovirus (Ad) E1-complementing cell lines which significantly reduce the presence of replication competent Ad (RCA) and can serve for the large scale production of infectious E1-deleted adenoviral particles that may be used for the treatment human patients as for example in gene therapy. As well the invention relates to a method for the large scale production of recombinant infectious adenoviral particles harboring an exogenous sequence of interest and to a RCA-free stock of infectious adenoviral particles. The invention further relates to a recombinant vector for transfecting an eukaryotic cell line in order to construct Ad E1-complementing cell lines which significantly reduce the presence of RCA and to a method therefor.

REFERENCES:
Lochmuller, H. et al., 1994, Human Gene Therapy 5:1485-1491.
Imler, J.-L. et al., 1996, Gene Therapy 3:75-84.
Acsadi, G. et al., 1995, J. Mol. Med. 73:165-180.
Graham, Frank L. et al., 1991, Methods in Molecular Biology 7:109-128.
Massie, Bernard, 1988, BioTechniques, vol. 6, No. 7, pp. 616-629.
Imler, J-L et al., 1995, Gene Therapy 2:263-268.
Schaak, Jerome et al., 1995, Journal of Virology, vol. 69, No. 6, pp. 3920-3923.
Babiss, Lee E. et al., 1983, Journal of Virology, vol. 46, No. 2, pp. 454-465.
Acsadi, Gyula et al., 1994, Human Molecular Genetics, vol. 3, No. 4, pp. 579-584.
Zabner, Joseph et al., 1993, Cell, 75:207-216.
Mittereder, Nanette et al., 1994, Human Gene Therapy 5:717-729.
Boucher, Richard C. et al., 1994, Human Gene Therapy 5:615-639.
Engelhardt, John F. et al., 1993, Nature Genetics 4: 27-34.
Trapnell, Bruce C. et al., 1994, Current Opinion in Biotechnology 5:617-625.
Wilmott, Robert W. et al., 1996, Human Gene Therapy 7:301-318.
Massie, Bernard et al., 1995, Biotechnology 13:602-608.
Graham, F.L. et al., 1977, J. Gen. Virol. 36:59-72.
Bett, Andrew J. et al., 1994, Proc. Natl. Acad. Sci. USA 91:8802-8806.
Gunning, Peter et al., 1987, Proc. Natl. Acad. Sci. USA 84:4831-4835.
Mulligan, Richard C., 1993, Science 260:926-932.
Sambrook et al., 1989, Cold Spring Harbor Laboratory Press, pp. 21-104.

Affiliated with

Also associated with

Rating

Adenovirus E1-complementing cell lines does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Adenovirus E1-complementing cell lines, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Adenovirus E1-complementing cell lines will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1370443