Adduct protection assay

Details Adduct protection assay Adduct protection assay

1995-06-07

1998-03-24

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 436164, 436172, 436 56, 436 63, 436 94, 436 86, C12Q 168, G01N 2100, G01N 2176, G01N 3300

Patent

active

057311480

ABSTRACT:
The present invention features an adduct protection assay involving the use of a labelled binding partner and a signal altering ligand. The signal altering ligand can preferentially alter the ability of label which is not part of a binding partner:analyte complex to produce a detectable signal, compared to its ability to alter signal produced from label which is part of a binding partner:analyte complex. The presence or amount of analyte can be determined by detecting the signal produced from unaltered label. The adduct protection assay is very versatile. For example, alteration of signal can be carried out under a wide range of conditions (e.g., pH, temperature, and ionic strength), and both label alteration and signal triggering can be carried out at essentially constant temperature to achieve a high degree of sensitivity.

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