Additives for use in cycling probe reactions

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 911, C12Q 168, C12P 1934

Patent

active

061365335

ABSTRACT:
A method for detecting a target nucleic acid molecule is provided, comprising the steps of (a) reacting a mixture comprising (i) a target nucleic acid molecule; (ii) a single-stranded nucleic acid probe containing a scissile linkage; (iii) an enzyme capable of cleaving the probe portion of a double-stranded target-probe complex at the scissile linkage; and (iv) ribosomal protein and/or spermine, under conditions and for a time sufficient to allow the target nucleic acid and probe to hybridize to each other and form a double-stranded target-probe complex, followed by cleavage of the probe and cycling of the target to a new uncleaved probe, such that one or more portions of the cleaved nucleic acid probe are released from the target-probe complex; and (b) determining whether cleaved portions of the nucleic acid probe are produced, and thereby detecting the presence of the target nucleic acid.

REFERENCES:
patent: 4876187 (1989-10-01), Duck et al.
patent: 5011769 (1991-04-01), Duck et al.
patent: 5403711 (1995-04-01), Walder et al.
patent: 5660988 (1997-08-01), Duck et al.
patent: 5731146 (1998-03-01), Duck et al.
Duck et al., "Probe Amplifier System Based on Chimeric Cycling Oligonucleotides," BioTechniques 9(2): 142-147, 1990.
Modrusan et al., "Spermine-mediated improvement of cycling probe reaction," Molecular and Cellular Probes 12: 107-116, 1998.
Pingoud et al., "Effect of Polyamines and Basic Proteins on Cleavage of DNA by Restriction Endonucleases," Biochemistry 23: 5697-5703, 1984.
Wetmur, "DNA Probes: Applications of the Principles of Nucleic Acid Hybridization," Critical Reviews in Biochemistry and Molecular Biology 26(3/4): 227-259, 1991.
Altwegg, "General problems associated with diagnostic applications of amplification methods," Journal of Microbiological Methods 23: 21-30, 1995.
Carrino and Lee, "Nucleic acid amplification methods," Journal of Microbiological Methods 23: 3-20, 1995.
Germann and Telenti, "Nucleic acid amplification methods in diagnostic virology," Journal of Microbiological Methods 23: 31-39, 1995.
Kitchin et al., "Avoidance of false positives," Nature 344: p. 201, 1990.
Kwok and Higuchi, "Avoiding false positives with PCR," Nature 339: 237-238, 1989.
Niederhauser et al., "Reliability of PCR Decontamination Systems," PCR Methods and Applications 4(2): 117-123, 1994.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Additives for use in cycling probe reactions does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Additives for use in cycling probe reactions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Additives for use in cycling probe reactions will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1962134

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.