Adaptors, and synthesis and cloning of proinsulin genes

Details Adaptors, and synthesis and cloning of proinsulin genes Adaptors, and synthesis and cloning of proinsulin genes

1983-12-16

1988-12-20

Nucker, Christine M.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 68, 4351723, 935 6, 935 13, 935 23, C07H 512, C12N 1500

Patent

active

047926023

ABSTRACT:
A human-like proinsulin gene and its analogs, have been synthesized by a combination of chemical and enzymatic methods. A number of different human-like proinsulin gene analogs with altered C-chains have also been designed and can be readily constructed as described. As a part of the strategy, an adaptor for trimming DNA has been designed, synthesized and used to recover the A-chain insulin gene with the desired sequence from a hybrid plasmid; a related adaptor for trimming DNA has been designed to shorten the C-chain gene or any gene. The synthetic proinsulin gene has been joined to a replicable cloning vehicle and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired human-like proinsulin gene.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4321365 (1982-03-01), Wu et al.
patent: 4431740 (1984-12-01), Bell et al.
Villa-Komaroff et al, A Bacterial clone synthesizing Pro-Insulin, Proc. Natl. Acad. Sci. U.S.A. vol. 75, 1978, pp. 3727-3731.
Roberts, Directory of Restriction Endonucleases, Methods in Enzymology vol. 68, 1979, pp. 27-41.
Rothstein et al., Synthetic Adaptors for Cloning DNA, Methods in Enzymology, vol. 68, 1978, pp. 98-109.
Brevet et al., Promotion of Insertions and Deletions by Translocating Segments of DNA Carrying Antibiotic Resistance Genes, in DNA Insertion Elements, Plasmids and Episomes, 1977, Cold Spring Harbor Labs.
Marians et al., Nature, 263: 744-748 (1976).
Heyneker et al., Nature, 263: 748-752 (1976).
Scheller et al., Science, 196: 177-180 (1977).
Bahl et al., Gene, 1:81-92 (1976).
Pribnow, Proc. Nat. Acad. Sci., 72: 784-788 (1975).
Polisky et al., Proc. Nat. Acad. Sci., 73: 3900-3905 (1976).
Itakura et al., Science, 198: 1056, 1066 (1977).
Goeddel et al., Nature, 281: 544-548 (1979).
Goeddel et al., Proc. Nat. Acad. Sci., :106-110 (1979).
Ullrich et al., Proceedings of the Symposium on Proinsulin, Insulin and C-Peptide, Tokushima, 12-14 Jul., 1978, pp. 20-26 (1976).
Crea et al., Proc. Nat. Acad. Sci., 75: 5765 (1978).
Vella-Konaroff et al, Proc. Nat. Acad. Sci., 75:3727 (1978).

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