Adaptor molecules for DNA and their application to synthesis of

Details Adaptor molecules for DNA and their application to synthesis of Adaptor molecules for DNA and their application to synthesis of

1982-09-29

1986-10-14

Hazel, Blondel

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

4351723, C07H 2104, C12N 1500

Patent

active

046173846

ABSTRACT:
Adaptor molecules have been prepared to comprise either start or stop signals for protein synthesis, in addition to recognition sites for restriction endonucleases. Separate adaptors may be used in a symmetrical duplex form. The start adaptor may include nucleotide base inserts to provide the correct reading frame of the triplet code in a DNA sequence with inappropriate reading frame. Insulin A-chain and B-chain genes of the human type, have been synthesized with the appropriate adaptor molecules provided on each end. The adapted DNA genes have been joined to replicable cloning vehicles and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired insulin gene.

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