Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-04-15
1999-03-30
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 9152, C12Q 168, C12N 1509
Patent
active
058887377
ABSTRACT:
An improvement in adaptor-based sequence analysis is provided that addresses the problems created by self-ligation of target polynucleotides that have complementary ends. The improvement includes preparation of target polynucleotides with dephosphorylated 5' strands and the use of adaptors having a 3' blocking group. In a preferred embodiment, adaptors are ligated to target polynucleotides by a single strand, 3' blocking groups are removed, the adjacent 5' hydroxyl of the target polynucleotide is phosphorylated, and the ligation of the adaptor is completed by treatment with a ligase.
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Albrecht Glenn
Brenner Sydney
Dubridge Robert B.
Gryaznov Sergei M.
McCurdy Sarah N.
Degen Nancy
Larson Thomas G.
Lynx Therapeutics, Inc.
Macevicz Stephen C.
Petithory Joanne R.
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