Adaptive baseline algorithm for quantitative PCR

Details Adaptive baseline algorithm for quantitative PCR Adaptive baseline algorithm for quantitative PCR

2008-04-22

2008-04-22

Zeman, Mary K (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C702S020000, C703S011000, C435S006120

Reexamination Certificate

active

07363168

ABSTRACT:
The invention relates to baseline subtraction algorithms developed to reduce tube-to-tube and cycle-to-cycle variabilities during real time PCR amplification. Particularly, the invention relates to algorithms for determining the threshold cycle for the first reliable detection of the amplified nucleic acid product. The invention also relates to computer programs comprising the algorithm and methods and/systems implementing the algorithm.

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Merriam-Webster On-Line Dictionary, available at http://www.m-w.com/cgi-bin/dictionary, accessed Jun. 6, 2005.
International Search Report of International Application No. PCT/US02/31144.
Leverkoehne Ina et al., Real-Time RT-PCR Quantitation Of MCLCA1 and MCLCA2 Reveals Differentially Regulated Expression In Pre-And Postnatal Murine Tissues,Histochemistry and Cell Biology, vol. 118, No. 1, Jul. 2002, pp. 11-17, XP002323027 ISSN: 0948-6143.
Supplementary European Search Report regarding EP 02763814.
Pfaffi, “A new mathematical model for relative quantification in real-time RT-PCR”; Nucleic Acids Research, 2001, vol. 29, No. 9 e45.

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