Adaptation of cell lines to suspension culture

Details Adaptation of cell lines to suspension culture Adaptation of cell lines to suspension culture

1976-11-03

1977-11-22

Rosen, Sam

Textiles: fiber preparation

C12K 900

Patent

active

040594858

ABSTRACT:
Densely packed cells growing rapidly in substrate-attached culture are gently released from the substrate by enzymatic treatment to form aggregates ranging from 20 .mu. to 500 .mu. in diameter. These aggregates are then transferred to fresh medium in agitated liquid suspension. The cells thereby continue to grow in aggregates whereby a normally anchorage-dependent estabished mammalian cell line is rapidly adapted to suspension culture.

REFERENCES:
patent: 3850748 (1974-11-01), Cook et al.
Willmer-Cells & Tissues in Culture vol. 1 (1965) pp. 495-496.

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