Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound having a 1-thia-5-aza-bicyclo
Reexamination Certificate
2007-03-27
2007-03-27
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound having a 1-thia-5-aza-bicyclo
C435S230000, C435S320100, C435S252330, C435S069100, C435S252300, C536S023200, C536S023100, C536S023700, C536S024100
Reexamination Certificate
active
10516587
ABSTRACT:
The subject of the present invention is to provide a β-lactam acylase protein having high activity, a gene coding for said β-lactam acylase protein, a recombinant vector having said gene, a transformant containing said recombinant vector, and a method of producing a β-lactam antibiotic such as amoxycillin using said β-lactam acylase. A β-lactam acylase gene ofStenotrophomonas maltophiliawas cloned, the DNA base sequence and the amino acid sequence expected therefrom was determined, and aStenotrophomonasβ-lactam acylase gene was obtained. This gene was found to code for a protein with a molecular weight of about 70 kDa and having β-lactam acylase activity, and could efficiently produce amoxycillin without being inhibited by phenylacetic acid, etc. Furthermore, by modification of the amino acid sequence, a protein which can more efficiently produce amoxycillin could be obtained.
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Kim et al., “Characterization of Glutaryl 7-ACA Acylase fromPseudomonas diminutaKAC-1,” J. Microbiology and Biotechnology (The Korean Society for Applied Microbiology), vol. 11, No. 3, pp. 452-457 (2001, Seoul, S. Korea).
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Mano Takumi
Nishi Akiko
Takano Masayuki
Yajima Kazuyoshi
Yokota Shin-ichi
Kaneka Corporation
Prouty Rebecca E.
Walicka Malgorzata A.
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