Acyl-peptide hydrolase and methods of production and use

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4352402, 4352523, 435212, 4353201, 4352542, 536 232, C12N 1557, C12N 1563, C12N 121, C12N 510

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active

055124674

ABSTRACT:
The invention discloses the amino acid sequence of Acyl-Peptide Hydrolase. The invention relates to a DNA molecule encoded for Acyl-Peptide Hydrolase, a vector incorporating the molecule, and the use of such vector to transform a host. The invention further relates to the expression by the host to produce Acyl-Peptide Hydrolase, and the use of APH to catalyze the hydrolysis of an N.sup..alpha. -acetylated peptide or protein or the reaction between an N.sup..alpha. -acetyl amino acid donor and an acceptor protein with a free .alpha.-NH.sub.2 group. The invention further relates to the use of the Acyl-Peptide Hydrolase-encoding sequences in the detection of cancer.

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