Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1995-07-31
1998-11-10
Grimes, Eric
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
536 232, C12N 910, C07M 2104
Patent
active
058342834
ABSTRACT:
This invention pertains to purified, biologically active acyl coenzyme A: cholesterol acyltransferase (ACAT) and to nucleic acid (DNA or RNA) encoding acyl coenzyme A:cholesterol acyltransferase. The nucleic acid, or a fragment thereof, may be ligated with an expression vector and transfected into cells to express acyl coenzyme A:cholesterol acyltransferase activity in intact cells and in cell-free extracts.
REFERENCES:
patent: 4652639 (1987-03-01), Stabinsky
patent: 5023243 (1991-06-01), Tullis
patent: 5075227 (1991-12-01), Hagen
patent: 5173408 (1992-12-01), Large, III et al.
patent: 5215915 (1993-06-01), Tiberi et al.
patent: 5217865 (1993-06-01), Myerowitz
patent: 5281520 (1994-01-01), O'Hara et al.
Babbitt et al., (1992) "Ancestry of the 4-Chlorobenzoate Dehalogenase: Analysis of Amino Acid Sequence Identities among Families of Acyl: Adenyl Ligases, Enoyl-CoA Hydratases/Isomerases, and Acyl-CoA Thioesterases," Biochemistry, vol. 31, 5594-5604.
Cadigan et al., (1988) "Isolation and Characterization of Chinese Hamster Ovary Cell Mutants Deficient in Acyl-Coenzyme A: Cholesterol Acyltransferase Activity, " Journal of Biological Chemistry, vol. 263(1), 274-282.
Cadigan, K.M., et al., (1989), "Isolation of Chinese Hamster Ovary Cell Lines Expressing Human Acyl-Coenzyme A/Cholesterol Acyltransferase Activity," The Journal of Cell Biology, vol. 108, No. 6, 2201-2210.
Cadigan et al., (1988) "A simple method for reconstitution of CHO cell and human fibroblast acyl coenzyme A: cholesterol acyltransferase activity into liposomes" Journal of Lipid Research, vol. 29(12), 1683-1692.
Cadigan et al., (1990) "Isolation and Characterization of Chinese Hamster Ovary Cell Mutants Defective in Intracellular Low Density Lipoprotein-Cholesterol Trafficking" The Journal of Cell Biology, vol. 110(2), 295-308.
Chang and Doolittle, (1983) "Acyl Coenzyme A:Cholesterol O-Acyltransferase," The Enzymes, vol. 16, 523-539.
Chang, C. and T.Y. Chang (1993) "Molecular Cloning and Functional Expression of Human Acyl-Coenzyme A:Cholesterol Acyltransferase (ACAT) cDNA", The FASEB Journal, vol. 7, No. 7, A1147, Abstract No. 551.
Chang et al., (1993), "Molecular Cloning and Functional Expression of Human Acyl-Coenzyme A:Cholesterol Acyltransferase cDNA in Mutant Chinese Hamster Ovary Cells", The Journal of Biological Chemistry, vol. 268, No. 28, 20747-20755.
Chauton et al., (1988) "Acyl-Coenyzme A:Cholesterol Acyltransferase Assay: Silica Gel Column Separation of Reaction Products" Analytical Biochemistry, vol. 173(2), 436-439.
Doolittle, (1981) Dissertation Abstracts International, vol. 24(4), 1429B.
FortKamp et al., (1986) "Cloning and Expression in Escherichia coli of a Synthetic DNA for Hirudin, the Blood Coagulation Inhibitor in the Leech" DNA, vol. 5(6), 511-517.
International Search Report issued during prosecution of PCT/US 93/09704, received May 26, 1994.
Jolly et al., (1982) "Isolation of a genomic clone partially encoding human hypoxanthine phosphoribosyltransferase," Proceedings of the National Academy of Sciences, vol. 79, 5038-5041.
Kinnunen et al., (1988) "Chemical Modification of Acyl-CoA:Cholesterol O-Acyltransferase. 1.) Idenitification of Acyl-CoA:Cholesterol O-Acyltransferase Subtypes by Differential Diethyl Pyrocarbonate Sensitivity," Biochemistry, vol. 27, No. 19, 7344-7350.
Little et al., (1992) "Ontogeny of acyl-CoA: cholesterol acyltransferase in rat liver, intestine, and adipose tissue" American Journal of Physiology, vol. 262(4), G599-G602.
Metheral et al., (1991) "A 25-Hydroxycholesterol-resistant Cell Line Deficient in Acyl-CoA: Cholesterol Acyltransferase," Journal of Biological Chemistry, vol. 266(19), 12734-12740.
O'Brien et al., (1992) "ACAT Inhibitors: A Potential New Approach to the Treatment of Hypercholesterolaemia and Atherosclerosis," Cardiovasculars, 507-526.
Schlid et al., (1990) "Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations" Proceedings of the National Academy of Sciences U.S.A., vol. 87(8), 2916-2920.
Spector et al., (1979) "Role of AcylCoenzyme A:Cholesterol O-acyltransferase in Cholesterol Metabolism," Progress in Lipids Research, vol. 18, No. 1, 31-53.
Schmitz et al. (1990) Biotechnology of Dyslipoproteinemias: Applications in Diagnosis and Control, C. Lenfant et al. (eds.) Raven Press, New York, pp. 79-89.
Young et al. (1983) Proc. Natl. Acad. Sci. USA 80, pp. 1194-1198.
Sturley ((1997) Current opinion in lipidology 8, pp. 167-173.
Diczfalusy et al. (1996) Arteriosclerosis, thrombosis and vascular biology 16, pp. 606-610.
Chang Catherine C. Y.
Chang Ta-Yuan
Grimes Eric
Slobodyansky Elizabeth
Trustees of Dartmouth College
LandOfFree
Acyl coenzyme A:cholesterol acyltransferase (ACAT) does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Acyl coenzyme A:cholesterol acyltransferase (ACAT), we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Acyl coenzyme A:cholesterol acyltransferase (ACAT) will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1515757