Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2006-02-07
2006-02-07
Eyler, Yvonne (Department: 1646)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S069500, C435S252300, C435S320100, C514S002600, C530S351000
Reexamination Certificate
active
06994990
ABSTRACT:
The present invention provides isolated highly active hedgehog proteins esterified with a fatty acid having from 14 to 20 carbon atoms at the N-terminal domain of the protein. The highly active hh proteins are particularly useful therapeutic agents for treating bone disorders and neurodegenerative diseases. Methods for obtaining the highly active modified hedgehog proteins are also provided.
REFERENCES:
patent: 5364839 (1994-11-01), Gerhart et al.
patent: 5844079 (1998-12-01), Ingham et al.
patent: 6444793 (2002-09-01), Pepinsky
patent: WO 93/00050 (1993-01-01), None
patent: WO 95/16035 (1995-06-01), None
patent: WO 96/16668 (1996-06-01), None
patent: WO 97/35607 (1997-10-01), None
patent: WO 98/02454 (1998-01-01), None
patent: WO 98/30576 (1998-07-01), None
Cha et al., PNAS 94(10577-10582)1997.
Pepinsky et al., Identification of a Palmitic Acid-modified Form of Human Sonic hedgehog. Journal of Biological Chemistry, May 29, 1998.
Bumcrot DA et al., Mol. Cell. Biol. 15(4)2294-303, 1995.
Bumcrot, et al., Proteolytic Processing Yields Two Secreted Forms of Sonic hedgehog, Molecular and Cellular Biology, vol. 15, No. 4, pp. 2294-2303 (1995).
Seytter, et al., Hydrophobic Modifications at the Amino-Terminal Cysteine of Recombinant Sonic Hedgehog Signaling Domain Dramatically Increase Activity. Bone, vol. 23, p. S563, Abstract (1998).
Pepinsky, et al., Identification of a Palmitic Acid-modified Form of Human Sonic Hedgehog, The Journal of Biological Chemistry, vol. 273, pp. 14037-14045 (1998).
Porter, et al., Cholesterol Modification of Hedgehog Signaling Proteins in Animal Development, Science, vol. 274, pp. 255-259 (1996).
Nakamura, et al., Induction of Osteogenic Differentiation by Hedgehog Proteins, Biochemical and Biophysical Research Communications, vol. 237, pp. 465-469 (1997).
Bumcrot, et al., Proteolytic Processing Yields Two Secreted Forms of Sonic hedgehog, Molecular and Cellular Biology, vol. 15, No. 4, pp. 2294-2303 (1995).
Miao, et al., Sonic Hedgehog Promotes the Survival of Specific CNS Neuron Populations and Protects These Cells from Toxic Insult In Vitro, The Journal of Neuroscience, vol. 17, No. 15, pp. 5891-5899 (1997).
Fietz, et al., The hedgehog gene family in Drosophila and vertebrate development, Development 1994 Supplement, pp. 43-51 (1994).
Hynes, et al., Induction of Midbrain Dopaminergic Neurons by Sonic Hedgehog, Neuron, vol. 15, pp. 35-44 (1995).
Hammerschmidt, The world according to hedgehog, TIG, vol. 13, No. 1, pp. 14-21 (1997).
Kinto, et al., Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation, FEBS Letters, vol. 404, pp. 319-323 (1997).
Cha, et al., Rhodopsin Kinase: Expression in baculovirus-infected insect cells, and characterization of post-translational modifications, Proc. Natl. Acad. Sci, vol. 94, pp. 10577-10582 (1997).
Asahina et al., 1996, “Human osteogenic protein-1 induces chondroblastic, osteoblastic, and/or adipocytic differentiation of clonal murine target cells”, Exp. Cell. Res. 222:38-47.
Bitgood et al., 1996, “Sertoli cell signaling by Desert hedgehog regulates the male germline”, Curr. Biol. 6:298-304.
Chiang et al., 1996, “Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function”, Nature 83:407-413.
Karaplis et al., 1994, “Lethal skeletal dysplasia from targeted disruption of the parathyroid hormone-related peptide gene”, Genes Dev. 8:277-289.
Lai et al., 1995, “Patterning of the neural ectoderm of Xenopus laevis by the amino-terminal product of hedgehog autoproteolytic cleavage”, Development 121:2349.
Perrimon, 1995, “Hedgehog and beyond”, Cell 80:517-520.
Smith, 1994, “Hedgehog, the floor plate, and the zone of polarizing activity”, Cell 76:193-196.
Vortkamp et al., 1996, “Regulation of rate of cartilage differentiation by Indian hedgehog and PTH-related protein”, Science 273:613-622.
Wozney et al., 1993, “Bone morphogenetic proteins and their gene expression”,Cellular and Molecular Biology of Bone(New York: Academic Press) pp. 131-167.
Lang Kurt
Leser Ulrike
Seytter Tilman
Brannock Michael
Curis, Inc.
Eyler Yvonne
Ropes & Gray LLP
LandOfFree
Active modified hedgehog proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Active modified hedgehog proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Active modified hedgehog proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3651913