Activation of equine oocytes

Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal – Via microinjection of a nucleus into an embryo – egg cell – or...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C800S014000, C600S591000, C435S325000, C435S375000

Reexamination Certificate

active

10411613

ABSTRACT:
A method for activating an equine oocyte comprising exposing oocyte to a medium containing a concentration of calcium of at least about 4 mM. Preferably the oocyte is exposed to this concentration of calcium during activation.

REFERENCES:
patent: 5471993 (1995-12-01), Yoches
patent: 5496720 (1996-03-01), Susko-Parrish
patent: 5945577 (1999-08-01), Stice
patent: WO 95/17500 (1995-06-01), None
Hinrichs, K et al, 1995, Activation of horse oocytes, Biol Reprod Mono, 1:319-324.
Galli, C et al, Aug. 2003, A clones horse born to its dam twin, Nature, 424:635.
Woods, GL et al., Aug. 2003, A mule clones from fetal cells by nuclear transfer, Science, 301:1063.
Li, X et al, 2000, Effects of different activation treatments on fertilization of horse oocytes by intracytoplasmic sperm injection, Journal of Reproduction and Fertility, 119:253-260.
Boquest, AC, et al., “Production of Cloned Pigs from cultured fetal fibroblast cells”, Biology of Reproduction, 66: 1283-1287 (2002).
Bos-Mikich, A, et al., “Meiotic and mitotic Ca+2 oscillations affect cell composition in resulting blastocysts”, Developmental Biology, 182:172-179 (1997).
Carneiro, GF, et al., “Quantification and distribution of equine oocyte cortical granules during meiotic maturation and after activation”, Molecular Reproduction and Development, 63:451-458 (2002).
Carneiro, G, et al., “Influence of insulin-like growth factor-I and its interaction with gonadotropins, estradiol, and fetal calf serum on in vitro maturation and parthenogenic development in equine oocytes”, Biology of Reproduction, 65:899-905 (2001).
Chong, HT, et al., “Effect of elevated Ca+2 concentration in fusion/activation medium on the fusion and development of porcine fetal fibroblast nuclear transfer embryos”, Molecular Reproduction and Development, 61:488-492 (2002).
Choi, YH, et al, “Production of Nuclear Transfer Horse embryos by piezo-driven injection of somatic cell nuclei and activation with stallion sperm cytosolic extract”, Biology of Reproduction, 67:561-567 (2002).
Choi, YH, et al., “Activation of cumulus-free equine oocytes: effect of maturation medium, calcium ionophore concentration and duration of cycloheximide exposure”, Reproduction, 122:177-183 (2001).
Choi, YH, et al., “Effect of co-culture with theca intrean on nuclear maturation of horse oocytes with low meiotic competence, and subsequent fusion and activation rates after nuclear transder”, Theriogenology, 57:1005-1011 (2002).
Guignot, F, et al., “Preliminary observations in in vitro development of equine embryo after ICSI”, Reprod. Nutr. Dev., 38:653-663 (1998).
Hinrichs, K, et al, “In vitro fertilization of in vitro-matured equine oocytes effect of maturation medium, duration of maturation, and sperm calcium ionophore treatment, and comparison with rates of fertilization in vivo after oviductal transfer”, Biology of Reproduction, 67:256-262 (2002).
Inoue, K, et al., “Improved postimplantation development of rabbit nuclear transfer embryos by activation with inositol 1,4,5-trisphosphate”, Cloning anf Stem Cells, 4(4):311-317 (2002).
Kato, H., et al., “Treatment of equine oocytes with A23187 after intracytoplasmic sperm injection”, Equine Vet. J. Suppl., 25:51-53 (1997).
Kitiyanant, Y, et al., “Somatic Cell cloning in Buffalo (Bubalus bubalis): effects of interspecies cytoplasmic recipients and activation procedures”, Cloning and Stem Cells, 3(3):97-104 (2001).
Li, X., et al., “In vitro development of horse oocytes reconstructed with the nuclei of fetal and adult cells”, Biology of Reproduction, 66:1288-1292 (2002).
Li, X., et al., “influence of co-culture during maturation on the developmental potential of equine oocytes fertilized by intracytoplasmic sperm injection (ICSI)”, Reproduction, 121:925-932 (2001).
Squires, EJ, “Maturation and fertilization of equine oocytes”, Reproductive Technology, 12(1):31-45 (1996).
Zhang, JJ, et al., “In vitro fertilization of horse follicular oocytes matured in vitro”, Molecular Reproduction and Development, 26:361-365 (1990).
Li, et al, “Effects of different activation treatments on fertilization of horse oocytes by intracytoplasmic sperm injection,” J. Reprod. and Fertility, 119:253-260 (2000).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Activation of equine oocytes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Activation of equine oocytes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Activation of equine oocytes will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3941788

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.