Activation of a siliceous carrier for enzyme immobilization

Details Activation of a siliceous carrier for enzyme immobilization Activation of a siliceous carrier for enzyme immobilization

1980-08-20

1983-05-17

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an...

252413, 252449, 435175, 435181, C12N 1114, C12N 1118, C12N 1106

Patent

active

043840450

ABSTRACT:
An improved immobilized enzyme composite is prepared by subjecting a siliceous carrier to an initial treatment with alkali, then acid, then reacting the carrier with an organosilane. The organosilane may then be coupled through a covalent coupling agent with an enzyme. The alkali-acid treatment provides an exceptionally high number of hydroxyl groups per unit volume, so that the treated carrier is especially useful in the preparation of high performance immobilized enzyme compositions, particularly by multi-layering immobilization, providing a very high amount of enzyme activity per unit volume. Multi-layering is accomplished by successive steps of bonding a difunctional covalent coupling agent to a previously immobilized enzyme layer, then bonding an additional layer of enzyme to the coupling agent. This may be repeated to apply as many layers as desired.

REFERENCES:
patent: 3519538 (1968-09-01), Messing et al.
patent: 3556945 (1971-01-01), Messing
patent: 3639558 (1972-02-01), Csizmas et al.
patent: 3669841 (1972-06-01), Miller
patent: 3830699 (1974-08-01), Zaborsky
patent: 4229534 (1980-10-01), Defilippi
patent: 4243692 (1981-01-01), Scholze et al.

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