Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Patent
1996-08-15
1999-01-12
Stockton, Laura L.
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
5483644, 5483711, C07D23500, C07D40512
Patent
active
058592598
ABSTRACT:
The disclosed invention is drawn to pyrazolinone derivatives, and methods of use thereof, for non-radioactive labeling of amine-functionalized molecules, especially biomolecules such as DNA, RNA, PNA, oligomers, carbohydrates, amino acids and peptides. Carboxyl-containing reporter groups such as biotin and fluorescein may be activated for nucleophilic addition to a primary or secondary amine moiety. Representative compounds of the invention include Dimethoxytritylbiotin-XPP, biotin-XPP, and fluorescein-XPP.
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Kremsky Jonathan N.
Sinha Nanda D.
PerSeptive Biosystems Inc.
Stockton Laura L.
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