Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1982-08-02
1985-01-15
Hazel, Blondel
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435188, 435810, 436547, G01N 3354, C12N 996
Patent
active
044938900
ABSTRACT:
A method for increasing the ability of apoglucose oxidase to combine with flavin adenine dinucleotide (FAD) and derivatives thereof to form active glucose oxidase by interacting apoglucose oxidase with an immunologically derived binding substance, e.g., an antibody or a fragment thereof, having a specific binding affinity for glucose oxidase. The apoglucose oxidase/anti-glucose oxidase immune complex is characterized by enhanced ability to combine with FAD and FAD-derivatives to yield glucose oxidase activity. The activation is particularly significant at temperatures elevated from room temperature, e.g., between 30.degree.-45.degree. C. An improved homogeneous specific binding assay method is provided for determining ligands wherein an FAD label is used and is monitored by its ability to combine with apoglucose oxidase to form active glucose oxidase by including anti-glucose oxidase in the reaction mixture.
REFERENCES:
patent: 4281061 (1981-07-01), Zuk et al.
patent: 4318982 (1982-03-01), Hornby et al.
Rose et al., Principles of Immunology, MacMillan Publishing Co., N.Y., (1979), pp. 49-52.
Green et al., J. Immun., vol. 104, pp. 1094-1100, (1970).
Hazel Blondel
Klawitter Andrew L.
Miles Laboratories Inc.
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