Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1996-11-27
1998-11-03
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435189, 43525235, 4353201, 536 231, 536 234, 536 237, 536 241, C12P 2102, C07H 2104, C12N 121, C12N 1567
Patent
active
058306951
ABSTRACT:
A 1 kbp length of the 5'-noncoding region of the gene encoding cytochrome P-450.sub.sca-2 in the actinomycete Streptomyces carbophilus has transcription promoter activity which is substrate inducible. When the 1 kbp region is shortened, transcription activity becomes constitutive and the shortened promoters can be used advantageously in expression systems, especially those expressing P-450.sub.sca-2 in the presence of ML-236B to produce pravastatin sodium.
REFERENCES:
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patent: 5466590 (1995-11-01), Sariaslani et al.
Database WPI, Section Ch, Week 9133, Derwent Publications Ltd., London, GB; Class B04, AN 91-241722 of JP 03 155 790 A (Yamanouchi Pharm. Co. Ltd.), 3 Jul. 1991.
I. Wantanabe et al, "Cloning, Characterization and expression of gene encoding cytochrome P-450.sub.sca-2 from Streptomyces carbophilus involved in production of pravastatin, a specific HMG-CoA reductase inhibitor", GENE, vol. 163, 1995, pp. 81-85.
Patel et al. "Phenobarbital & sulfonylurea-inducible operons encoding herbicide metabolizing cytochromes P-450 in Streptomyces griseoius" Gene 112 67-76, 1992.
Purification and characterization of cytochrome P-450.sub.sca from Streptomyces carbophilus, Eur. J. Biochem., 184, 707-713 (1989).
Serizawa Nobufusa
Watanabe Ichiro
Degen Nancy
Sankto Company, Limited
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