Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2007-03-27
2007-03-27
Vogel, Nancy (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
Reexamination Certificate
active
11042977
ABSTRACT:
AnActinobacillus succinogenesplasmid vector which provides a means to overexpress proteins inA. succinogenes. The plasmid can be transformed efficiently by electroporation, and replicates in a stable manner inA. succinogenes. The plasmid comprises at least one marker gene, operably linked to a first promoter functional inActinobacillus succinogenes, an origin of replication functional inActinobacillus succinogenes, a second promoter isolated fromActinobacillus succinogenes, and a cloning site downstream from the second promoter. Plasmids pLGZ901, pLGZ920, pLGZ921, and pLGZ922 are disclosed. The pckA gene polypeptide sequence and nucleic acid sequence ofActinobacillus succinogenes, including the promoter and ribosome binding site, is disclosed. Furthermore, a method for producing a recombinantActinobacillus succinogenesis described, including a method of transformation. Additionally, a recombinantActinobacillus succinogenesis disclosed and a method for producing succinate utilizing this recombinantActinobacillus succinogenesis described.
REFERENCES:
patent: 5143833 (1992-09-01), Datta et al.
patent: 5573931 (1996-11-01), Guettler et al.
patent: RE37393 (2001-09-01), Donnelly et al.
patent: 6420151 (2002-07-01), Eikmanns et al.
patent: 6455284 (2002-09-01), Gokarn et al.
patent: 2003/0087381 (2003-05-01), Gokarn et al.
patent: 2003/0113885 (2003-06-01), Lee et al.
West, S.E., et al., Gene 160:81-86(1995).
Park, D.H., et al., J. Bacteriol. 181:2403-2410(1999).
Lalonde et al. (Am. J. Vet. Res. 50:1957-1960(1989).
Brogan, J.M., et al., Gene 169:141-142(1996).
Frey, J., Res. Microbiol. 143:263-269(1992).
Craig, F.F., et al., J. Gen. Microbiol. 135:2885-2890(1989).
Dixon, L.G., et al., Plasmid 32:228-232 (1994).
Oswald, W., et al., FEMS Microbiol. Lett. 179:153-160(1999).
Wright, C. L., Plasmid37:65-79(1997).
Kehrenberg, C., et al., Antimicrob.Agents Chemother. 42:2116-2118(1998).
West, S.E., et al., Gene 160:87-88 (1995).
Galli, D.M., et al., Plasmid 36:42-48 (1996).
Ishii, H., et al., Nippon Juigaku Zasshi 52:1-9 (1990).
Lalonde, G., et al., Gene 85:243-246(1989).
Nakano, Y., et al., Gene 169:139-140(1996).
Laivenieks, M., et al., Appl. Environ. Microbiol. 63:2273-2280(1997).
Shen, T.L., et al., J. Mass Spectrom. 34:1154-1165 (1999).
Brown, A.J.H., et al., Biotechniques26:804-806 (1999).
van der Werf, M.J., et al., Arch. Microbiol. 167:332-342(1997).
Kim Pil
Laivenieks Maris
Vieille Claire
Zeikus J. Gregory
Board of Trustees of Michigan State University
McLeod Ian C.
Merritt Steven E.
Vogel Nancy
LandOfFree
Actinobacillus succinogenes shuttle vector and methods of use does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Actinobacillus succinogenes shuttle vector and methods of use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Actinobacillus succinogenes shuttle vector and methods of use will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3725357