Acremonium typhinum ATCC 74228 which exhibits endoproteolytic ac

Details Acremonium typhinum ATCC 74228 which exhibits endoproteolytic ac Acremonium typhinum ATCC 74228 which exhibits endoproteolytic ac

1994-03-24

1995-08-22

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435911, 4352541, 435814, 435820, 424 935, 424 9463, C12N 958, C12N 100, C12N 114, A01N 6300

Patent

active

054439817

ABSTRACT:
A crude membrane fraction of leaf sheath tissue of a grass endophytically infected by A. typhinum is produced. The extract exhibits endoproteolytic activity in the presence of detergent or after methanol precipitation. This activity is optimal at 35.degree.-40.degree. C. and pH 10-11 and is exhibited only when a reductant is present. The activity is associated with a first gel electrophoresis band having apparent molecular weight of 205,000 daltons when said extract is electrophoresed without prior boiling; however, when said extract is boiled prior to electrophoresis, there results a band having apparent molecular weight of 34,000 daltons. This band gives rise to polyclonal rabbit antibodies which are not cross react with proteinase K. The preferred source of Acremonium typhinum is ATCC 74228.

REFERENCES:
Lindstrom et al., Supplement to Plant Physiology, vol. 102, No. 1, May 1993, p. 113.
Lindstrom et al., Plant Physiol., vol. 102, pp. 645-650, 1993.
Lindstrom et al., Supplement to Plant Physiology, vol. 99, No. 1, 1992, p. 111.

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