Acidophilic enzymes

Details Acidophilic enzymes Acidophilic enzymes

2011-08-23

2011-08-23

Desai, Anand (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

C435S006120, C435S091520, C536S023200

Reexamination Certificate

active

08003357

ABSTRACT:
The present invention relates to enzymes having catalytic activity at a pH below 5.0. The present invention provides hydrolyzing enzymes obtainable from archaeobacteria, in detail to hydrolytic enzymes obtainable from the archaeobacteriumFerroplasma acidiphilum. In general, the present invention provides enzymes which are active and stable at acidic pH values, especially at pH values from 1 to 4, especially in the range of pH 2 to 3, obtainable fromFerroplasma acidiphilum, especially to an esterase, glycosidases and a DNA ligase. In addition to stability and activity at low pH values, the enzymes according to the present invention are all dependent on Fe2+for their catalytic activity.

REFERENCES:
patent: 5506137 (1996-04-01), Mathur et al.
DNA ligase, UniProt Accession No. Q6L195, created Dec. 21, 2004.
Database Geneseq, “Bacterial Polypeptide #10965” XP002416599, Database Accession No. ADS21932, Dec. 2, 2004.
Database UniProt, “Alpha Glucosidase (EC 3.2.1.20)”, XP002416600, Database Accession No. Q5K3Q3, Feb. 15, 2005.
Database UniProt, “Carboxylesterase” XP002416601, Database Accession No. Q41TF6, Sep. 27, 2005.
Database UniProt, “Alpha-D-Glucoside Glucohydrolase 1 (EC 3.2.1.20)”, XP002416602, Database Accession No. Q2PCE3, Feb. 7, 2006.
Database UniProt, “Alpha-D-Glucoside Glucohydrolase 2 (EC 3.2.1.20)”, XP002416603, Database Accession No. Q2PCE2, Feb. 7, 2006.
Database UniProt, “Mandelate Racemase/Muconate Lactonizing Enzyme”, XP002416604, Database Accession No. Q41V65, Sep. 27, 2005.
Database UniProt, “Acetyl Esterase”, XP002416605, Database Accession No. Q6KZ86, Jul. 5, 2004.
Manuel Ferrer et al., “A Novel Alpha-Glucosidase from the Acidophilic Archaeon Ferroplasma Acidiphilum Strain Y with High Transglycosylation Activity and an Unusual Catalytic Nucleophile”,Biochemical Journal, vol. 391, No. 2, Oct. 2005, pp. 269-276, XP002416589 ISSN: 0264-6021.
O.V. Golyshina et al., “Ferroplasma Acidiphilum Gen. Nov., Sp., Nov., An Acidophilic, Autotrophic, Ferrous-Iron-Oxidizing, Cell-Wall-Lacking, Mesophilic Member of the Ferroplasmaceae Fam. Nov., Comprising a Distinct Lineage of the Archaea”,International Journal of Systematic and Evolutionary Microbiology, vol. 50, No. 3, 2000, pp. 997-1006. XP001117770 ISSN: 1466-5026.
O.V. Golyshina et al., “The ‘pH Optimum Anomaly’ of Intracellular Enzymes of Ferroplasma Acidiphilum”,Environmental Microbiology, vol. 8, No. 3, Mar. 2006, pp. 416-425. XP002416590 ISSN: 1462-2912.
Bertus Van Den Burg, “Extremophiles as a Source for Novel Enzymes”,Current Opinion in Microbiology, vol. 6, No. 3, Jun. 2003, pp. 213-218. XP002416591 ISSN: 1369-5274.
Manuel Ferrer et al., “A Purple Acidophilic Di-Ferric DNA Ligase from Ferroplasma”,PNAS, Jul. 1, 2008, vol. 105, No. 26, pp. 8878-8883.

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