Acidic phospholipase, production and methods using thereof

Details Acidic phospholipase, production and methods using thereof Acidic phospholipase, production and methods using thereof

1999-04-20

2000-10-03

Slobodyansky, Elizabeth

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435197, 4352541, C12Q 134, C12N 918, C12N 114

Patent

active

061271373

ABSTRACT:
An acidic phospholipase is obtained from a strain of the genus Hyphozyma. It is able to hydrolyze both fatty acyl groups in intact phospholipid. Advantageously, it has no lipase activity and is active at very low pH; these properties make it very suitable for use in oil degumming, as enzymatic and alkaline hydrolysis (saponification) of the oil can both be suppressed. The phospholipase is not membrane bound, making it suitable for commercial production and purification.

REFERENCES:
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Ichimasa et al. Agric. Biol. Chem., vol. 49, No. 4, pp. 1083-1089 (1985).
Lee et al. The Journal of Biological Chemistry, vol. 269, No. 31, pp. 19725-19730 (1994).
Kuwabara et al. Agric. Biol. Chem., vol. 52, No. 10, pp. 2451-2458 (1988).

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