Acidic agar gel electrochromatography of hemoglobins

Details Acidic agar gel electrochromatography of hemoglobins Acidic agar gel electrochromatography of hemoglobins

1979-06-01

1980-06-24

Prescott, Arthur C.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

23230B, 424 12, G01N 3316, G01N 2726

Patent

active

042093732

ABSTRACT:
The common hemoglobin variants A, A.sub.2, F, S, and C are discretely separated by electrochromatography under acidic conditions on an agar gel which utilizes an agar gel having a wet thickness of from about 0.1 to about 0.5 mm. and a potential of from about 60 to about 110 volts. The gel buffer is a citrate buffer having a citrate concentration of from about 0.04 to about 0.065 M and a pH of from about 6.0 to about 6.5. The well buffer is a citrate buffer having a citrate concentration of from about 0.055 to about 0.065 M and a pH of from about 6.0 to about 6.5.

REFERENCES:
patent: Re24752 (1959-12-01), Ressler
patent: 3497437 (1970-02-01), Louderback et al.
patent: 3558459 (1971-01-01), Granstrand et al.
patent: 3607695 (1971-09-01), Schneider
patent: 3692654 (1972-09-01), Svendsen

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