Acid fungal protease in fermentation of insoluble starch...

Sugar – starch – and carbohydrates – Processes – Carbohydrate manufacture and refining

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S093000

Reexamination Certificate

active

08075694

ABSTRACT:
The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.

REFERENCES:
patent: 4092434 (1978-05-01), Yoshizumi et al.
patent: 4514496 (1985-04-01), Yoshizumi et al.
patent: RE32153 (1986-05-01), Tamura et al.
patent: 4587215 (1986-05-01), Hirsh
patent: 4863864 (1989-09-01), Ashikari et al.
patent: 5093257 (1992-03-01), Gray
patent: 5231017 (1993-07-01), Lantero et al.
patent: 5278059 (1994-01-01), Sugimoto et al.
patent: 5545587 (1996-08-01), Sugimoto et al.
patent: 5736499 (1998-04-01), Mitchinson et al.
patent: 5874276 (1999-02-01), Fowler et al.
patent: 5958739 (1999-09-01), Mitchinson et al.
patent: 6022725 (2000-02-01), Fowler et al.
patent: 6093562 (2000-07-01), Bigard-Frantzen et al.
patent: 6254914 (2001-07-01), Singh et al.
patent: 6255115 (2001-07-01), Beijersbergen et al.
patent: 6268328 (2001-07-01), Mitchinson et al.
patent: 6352851 (2002-03-01), Nielsen et al.
patent: 6408105 (2002-06-01), Maruo
patent: 6436888 (2002-08-01), Svendsen et al.
patent: 6566125 (2003-05-01), Johnston et al.
patent: 6605458 (2003-08-01), Hansen et al.
patent: 6768001 (2004-07-01), Saloheimo et al.
patent: 6867031 (2005-03-01), Bigard-Frantzen et al.
patent: 6899910 (2005-05-01), Johnston et al.
patent: 6936110 (2005-08-01), Van Thorre
patent: 6936294 (2005-08-01), Matthews et al.
patent: 2005/0266543 (2005-12-01), Dunn-Coleman et al.
patent: 0 171 218 (1985-07-01), None
patent: 0 215 594 (1986-08-01), None
patent: WO 84/02921 (1984-08-01), None
patent: WO 92/00381 (1992-01-01), None
patent: WO 96/23874 (1996-08-01), None
patent: WO 96/39528 (1996-12-01), None
patent: WO 99/28488 (1999-06-01), None
patent: WO 99/34011 (1999-07-01), None
patent: WO 03/066826 (2003-08-01), None
patent: WO 03/068976 (2003-08-01), None
patent: WO 2004/080923 (2004-09-01), None
patent: WO 2004/081193 (2004-09-01), None
patent: WO 2004/106533 (2004-12-01), None
patent: WO 2004/111218 (2004-12-01), None
patent: WO 2004/113551 (2004-12-01), None
patent: WO 2005/001064 (2005-01-01), None
patent: WO 2005/003337 (2005-01-01), None
patent: WO 2005/052148 (2005-06-01), None
patent: WO 2005/087938 (2005-09-01), None
patent: WO 2006/073839 (2006-07-01), None
Allison, Daniel S. et al., << Transformation of the thermophilic fungusHumicola griseavar.thermoideaand overproduction ofHumicola glucoamylase, >> Current Genetics, vol. 21, pp. 225-229, 1992.
Altschul et al., “Basic Local Alignment Statistics, Methods in Enzymology”, V. 266, pp. 460-480 (1993).
Ashikari, Toshihiko et al., <<RhizopusRaw-Starch-Degrading Glucoamylase : Its Cloning and Expression in Yeast, >> Agric. Biol. Chem., vol. 50, No. 4, pp. 957-964, 1986.
Bajar, Aslam, et al., “Identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor, ”Proc. Natl. Acad. Sci. USA, vol. 88, pp. 8208-8212, Sep. 1991.
Boel, E. et al., << Glucoamylases G1 and G2 fromAspergillus nigerare synthesized from two different but closely related mRNAs, >> The EMBO Journal, vol. 3, No. 5, pp. 1097-1102, 1984.
Boel, E. et al., << Two different types of intervening sequences in the glucoamylase gene fromAspergillus niger, >> The EMBO Journal, vol. 3, No. 7, pp. 1581-1585, 1984.
Brigidi, DeRossi, Bertarini, Riccardi and Matteuzzi, (1990),FEMS Microbiol. Lett.67: 135-138.
Campos et al., “Purification and Characterization of a Glucoamylase fromHumicola grisea,” Applied and Envir. Micro., Jun. 1995, p. 2436-2438.
Chen, Hsiu-mei et al., “Identification and elimination by site-directed mutagenesis of thermolabile aspartyl bonds inAspergillus awamoriglucoamylase,”Protein Engineering, vol. 8, No. 6, pp. 575-582, 1995.
Chen, Hsiu-mei et al., << Effect of replacing helical glycine residues with alanines on reversible and irreversible stability and production ofAspergillus awamoriglucoamylase,Protein Engineering, vol. 9, No. 6, pp. 499-505, 1996.
Chen, Frank Y. et al., “Regulation of mammalian ribonucleotide reductase R1 mRNA stability is mediated by a ribonucleotide reductase R1 mRNA 3′-untranslated region cis-trans interaction through a protein kinase C-controlled pathway, ”Biochem. J., vol. 302, pp. 125-132, 1994.
Database EMBL pp. 1-2, Mar. 1, 2001, Delgado-Jarana et al., “Putative aspartate protease”, retrieved from EBI Datagase accession No. Q9HDT6.
Database EMBL pp. 1-2, Oct. 25, 2004 Viterbo et al., “Aspartyl protease” retrieved from EBI Database accession No. Q641D0.
Database EMBL pp. 1-3, Dec. 7, 2005, Mantyla, “Hypocrea jecorinaproA gene for aspartic protease, exons 1-2, strain QM6a”, retrieved from EBI Database accession No. AM168137.
Dayhoff et al., “A Model of Evolutionary Change in Proteins,”Atlas of Protein Sequence and Structure5:Suppl. 3 (National Biomedical Research Foundation, Washington, D.C. (1978).
Eneyskaya et al., “Acid protease from richoderma reesei : limited proteolysis of fungal carbohydrases,”Applied Microbiology and Biotechnology, V. 52, 1999, pp. 226-231.
Fernandez-Abalos et al., “Posttranslational processing of the xylanase Xys1L fromStreptomyces halstediiJM8 is carried out by secreted serine proteases,”Microbiology, 2003, 149, 1623-1632.
Finkelstein, David B. et al., “Biotechnology of Filamentous Fungi,” Technology and Products, Butterworth-Heinemann, David Finkelstein, ed., pp. 113-156, 1992.
Goldman, et al., “Transformation ofTrichoderma harzianumby high-voltage electric pulse,”Current Genetics, 17:169-174, 1990.
Han et al.m “Amylolysis of Raw Corn byAspergillus nigerfor Simultaneous Ethaol Fermentation,”Biot. & Bioeng., V30, pp. 225-232 (1987).
Hata, Yoji et al., << The Glucoamylase cDNA fromAspergillus oryzae: Its Cloning, Nucleotide Sequence, and Expression inSaccharomyces cerevisiae, >> Agric. Biol. Chem., vol. 55, No. 4, pp. 941-949, 1991.
International Search Report for PCT/US2005/046474, filed Dec. 21, 2005.
Jensen, Bo et al., << Purification of extracellular amylotic enzymes from the thermophilic fungusThermomyces lanuginosus, Can. J. Microbiol., vol. 34, pp. 218-223.
Ponnampalam et al., “Effect of Germ and Fiber Removal on Production of Ethanol from Corn,”Applied Biochem and Biotech., V. 113-116, 2004.
Singh et al., “An Enzymatic Process for Corn Wet Milling,”Advances in Food and Nutrition Res., V. 48, pp. 151-171, 2004.
Singh et al., “Recovery of Fiber in the Corn Dry-Grind Ethanol Process : A Feedstock for Valuable Coproducts,”Cereal Chemistry, V. 76,N.6, 1999.
Smith, et al.,“Comparison of Biosequences,”Adv. Appl. Math. 2:482-489, 1981.
Takahashi et al., “Different Behavior towards Raw Starch of Three Forms of Glucoamylase from aRhizopusSp.,”J. Biochem., 663- 671, (1985).
Taylor, Pamela M. et al., << Some Properties of a Glucoamylase Produced by the Thermophilic FungusHumicola lanuginosa, >> Carbohydrate Research, vol. 61, pp. 301-308, 1978.
Tosi, Luis Ricardo Orsini et al., << Purification and characterization of an extracellular glycoamylase from the thermophilic fungusHumicola griseavar.thermoidea, >> Can J. Microbiol., vol. 39, pp. 846-855, 1993.
Ueda et al., “Production of Ethanol from Raw Cassava Starch by a Nonconventional Fermentation Method,”Biotechnology and Bioengineering, Vol. XXIII, 291-299, (1981).
Wang et al., “Co

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Acid fungal protease in fermentation of insoluble starch... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Acid fungal protease in fermentation of insoluble starch..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Acid fungal protease in fermentation of insoluble starch... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4302858

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.