Acid dye staining method

Details Acid dye staining method Acid dye staining method

1992-06-23

1994-02-08

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041801, 20429900R, 8506, 436 86, 436166, G01N 2726, G01N 27447, G01N, G01N, G01N 2175

Patent

active

052845605

ABSTRACT:
An acid dye staining method for electrophoretically separated fractions on a supporting matrix characterized by fixing the supporting matrix after electrophoresis with an aqueous solution containing a lower alcohol and an organic acid, followed by staining with an acid dye solution mixed with at least one specified acid, can reduce a time required for staining remarkably and improve the sensitivity greatly.

REFERENCES:
patent: 4023933 (1977-05-01), Bradford et al.
patent: 4405720 (1983-09-01), Merril
patent: 5064768 (1991-11-01), Ebata et al.
Analytical Biochemistry 152, pp. 308-313, 1986.
Analytical Biochemistry 20, pp. 150-154, 1967.
Anthony T. Andrews "Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications" 2nd ed. 91986) p. 28.

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