Achromobacter protease I gene and gene product thereof

Details Achromobacter protease I gene and gene product thereof Achromobacter protease I gene and gene product thereof

1990-02-20

1993-09-28

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4353201, 435220, 435824, 536 232, 935 14, C12P 2102, C12N 1557, C12N 1563, C12N 1531

Patent

active

052485994

ABSTRACT:
Disclosed is a DNA sequence containing a DNA segment coding for Achromobacter protease I (API) or variants thereof (referred to as T-API); a recombinant DNA constructed by introducing the DNA sequence in an expression vector so as to express the T-API; a transformant bearing the recombinant DNA; a process for producing the API which comprises cultivating the transformant, accumulating the T-API in a culture product, and recovering the same: and a protein of T-API. The cells transfected or transformed with the DNA sequence of the present invention allow for the production of a large amount of precursor protein of the T-API or the mature peptide.

REFERENCES:
patent: 4581332 (1986-04-01), Soejima et al.
patent: 4624926 (1986-11-01), Inouye et al.
Young, R. A., et al. Proc. Nat'l Acad. Sci., USA. 80: 1194-1198 (1983).

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