Multicellular living organisms and unmodified parts thereof and – Method of using a transgenic nonhuman animal in an in vivo...
Reexamination Certificate
2001-08-14
2004-05-11
Paras, Peter (Department: 1632)
Multicellular living organisms and unmodified parts thereof and
Method of using a transgenic nonhuman animal in an in vivo...
C800S008000, C800S018000
Reexamination Certificate
active
06734337
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates generally to the field of fat metabolism and weight control. More specifically, the present invention relates to the role of the ACC2 isoform of acetyl-CoA carboxylase in regulating fatty acid accumulation and oxidation.
2. Description of the Related Art
Acetyl-CoA carboxylase (ACC), a biotin-containing enzyme, catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA, an intermediate metabolite that plays a pivotal role in the regulation of fatty acid metabolism. It has been found that malonyl-CoA is a negative regulator of carnitine palmitoyltransferase I (CPTI, a component of the fatty-acid shuttle system that is involved in the mitochondrial oxidation of long-chain fatty acids. This finding provides an important link between two opposed pathways-fatty-acid synthesis and fatty-acid oxidation. Thus, it is possible to interrelate fatty acid metabolism with carbohydrate metabolism through the shared intermediate acetyl-CoA, the product of pyruvate dehydrogenase. Consequently, the roles of malonyl-CoA in energy metabolism in lipogenic (liver and adipose) and non-lipogenic (heart and muscle) tissues has become the focus of many studies.
In prokaryotes, acetyl-CoA carboxylase is composed of three distinct proteins-the biotin carboxyl carrier protein, the biotin carboxylase, and the transcarboxylase. In eukaryotes, however, these activities are contained within a single multifunctional protein that is encoded by a single gene.
In animals, including humans, there are two isoforms of acetyl-CoA carboxylase expressed in most cells, ACC1 (M
r
~265,000) and ACC2 (M
r
~280,000), which are encoded by two separate genes and display distinct tissue distribution. Both ACC1 and ACC2 produce malonyl-CoA, which is the donor of the “C
2
-units” for fatty acid synthesis and the regulator of the carnitine palmitoyl-CoA shuttle system that is involved in the mitochondrial oxidation of long-chain fatty acids. Hence, acetyl-CoA carboxylase links fatty acid synthesis and fatty acid oxidation and relates them with glucose utilization and energy production, because acetyl-CoA, the substrate of the carboxylases, is the product of pyruvate dehydrogenase. This observation, together with the finding that ACC1 is highly expressed in lipogenic tissues such as liver and adipose and that ACC2 is predominantly expressed in heart and skeletal muscle, opened up a new vista in comparative studies of energy metabolism in lipogenic and fatty acid-oxidizing tissues.
Diet, especially a fat-free one, induces the synthesis of ACC's and increases their activities. Starvation or diabetes mellitus represses the expression of the Acc genes and decreases the activities of the enzymes. Earlier studies addressed the overall activities of the carboxylases with specific differentiation between ACC1 and ACC2. Studies on animal carboxylases showed that these enzymes are under long-term control at the transcriptional and translational levels and short-term regulation by phosphorylation/dephosphorylation of targeted Ser residues and by allosteric modifications induced by citrate or palmitoyl CoA.
Several kinases have been found to phosphorylate both carboxylases and to reduce their activities. In response to dietary glucose, insulin activates the carboxylases through their dephosphorylation. Starvation and/or stress lead to increased glycogen and epinephrine levels that inactivate the carboxylases through phosphorylation. Experiments with rats undergoing exercises showed that their malonyl-CoA and ACC activities in skeletal muscle decrease as a function of exercise intensity thereby favoring fatty acid oxidation. These changes are associated with an increase in AMP-kinase activity. The AMP-activated protein kinase (AMPK) is activated by a high level of AMP concurrent with a low level of ATP through mechanism involving allosteric regulation and phosphorylation by protein kinase (AMP kinase) in a cascade that is activated by exercise and cellular stressors that deplete ATP. Through these mechanisms, when metabolic fuel is low and ATP is needed, both ACC activities are turned off by phosphorylation, resulting in low malonyl-CoA levels that lead to increase synthesis of ATP through increased fatty acid oxidation and decreased consumption of ATP for fatty acid synthesis.
Recently, it was reported that the cDNA-derived amino acid sequences of human ACC1 and ACC2 share 80% identity and that the most significant difference between them is in the N-terminal sequence of ACC2. The first 218 amino acids in the N-terminus of ACC2 represent a unique peptide that includes, in part, 114 of the extra 137 amino acid residues found in this isoform. Polyclonal antibodies raised against the unique ACC2 N-terminal peptide reacted specifically with ACC2 proteins derived from human, rat, and mouse tissues. These findings made it possible to establish the subcellular localization of ACC1 and ACC2 and to later demonstrate that ACC2 is associated with the mitochondria and that the hydrophobic N-terminus of the ACC2 protein plays an important role in directing ACC2 to the mitochondria. ACC1, on the other hand, is localized to the cytosol.
Although these findings and the distinct tissue distribution of ACC1 and ACC2 suggest that ACC2 is involved in the regulation of fatty acid oxidation and that ACC1 is involved in fatty acid synthesis primarily in lipogenic tissues, they do not provide direct evidence that the products of the genes ACC1 and ACC2 have distinct roles.
These distinctions between the two ACC isoforms could not have been predicted prior to the generation of the Acc2 knockout mouse described herein. Moreover, malonyl-CoA, the product of the ACC1 and ACC2, seems to be present in the liver and possibly in other tissues in two separate pools that do not mix and play distinct roles in the physiology and metabolism of the tissues. Malonyl-CoA, the product of ACC1, is involved in fatty acid synthesis as the donor of “C2-carbons.” On the other hand, malonyl-CoA, the product of ACC2, is involved in the regulation of the carnitine palomitoyl CoA shuttle system, hence in the oxidation of fatty acids. This functional distinction between the roles of the products of ACC1 and ACC2 based on the results obtained with the Acc2 mice was not expected nor could it have been predicted prior to this study.
Moreover, the current study demonstrates that ACC2, through its product malonyl-CoA, is potentially an important target for the regulation of obesity. Inhibition of ACC2 would reduce the production of malonyl-CoA, leading to continual fatty acid oxidation and energy production. This continual oxidation of fatty acid would be achieved at the expense of freshly synthesized fatty acids an d triglycerides and of body fat accumulated in the adipose and other fatty tissues leading to reduced body fat.
The prior art is deficient in an understanding of the separate roles ACC1 and ACC2 have in the fatty acid metabolic pathways. The prior art is also deficient in assigning the differential roles of the malonyl-CoA generated by ACC1 versus that generated by ACC2 in regulating fatty acid metabolism. Also, the prior art is deficient in transgenic knockout mice generated to lack ACC2 and methods of using these transgenic mice. The present invention fulfills this long-standing need and desire in the art.
SUMMARY OF THE INVENTION
Malonyl-CoA (Ma—CoA), generated by acetyl-CoA carboxylases ACC1 and ACC2, is the key metabolite in the regulation of fatty acid (FA) metabolism. Acc1
−/−
mutant mice were embryonically lethal, possibly due to a lack of “C
2
-units” for the synthesis of fatty acid needed for biomembrane synthesis. Acc2
−/−
mutant mice bred normally and had normal life spans. Acc2
−/−
mice fed normal diets did not accumulate fat in their livers as did the wild-type mice and overnight fasting resulted in a 5-fold increase in ketone bodies production, indicating higher fatty acid oxidation. ACC1 and fatty acid synthase activities and malonyl-CoA co
Abu-Elheiga Lutfi
Matzuk Martin
Wakil Salih J.
Adler Benjamin Aaron
Paras Peter
Research Development Foundation
LandOfFree
Acetyl-coenzyme A carboxylase 2 as a target in the... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Acetyl-coenzyme A carboxylase 2 as a target in the..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Acetyl-coenzyme A carboxylase 2 as a target in the... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3205038