Acetic anhydride activation for C-terminal protein sequencing

Details Acetic anhydride activation for C-terminal protein sequencing Acetic anhydride activation for C-terminal protein sequencing

1994-09-08

1995-11-21

Schain, Howard E.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Chemical aftertreatment, e.g., acylation, methylation, etc.

436 89, 436 90, 530408, C07K 110, G01N 3368

Patent

active

054688434

ABSTRACT:
A method is provided for C-terminal sequencing of a protein or peptide. An important feature of the method is the formation of an oxazolone moiety at the C-terminus of a protein or peptide by treatment with acetic anhydride under basic conditions followed by conversion of the oxazolone to a thiohydantoin moiety by treatment with thiocyanate under acidic conditions. Yields of thiohydantoin are further enhanced by delivering thiocyanate as the conjugate acid of a sterically hindered alkylammnonium cation.

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patent: 5304497 (1994-04-01), Boyd et al.
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