Acetate selected bacillus thuringiensis and the method of use

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

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4351721, 4351723, 435242, 4352525, 435832, A01N 6300, C12N 1501, C12N 122, C12N 300

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active

049504711

ABSTRACT:
Methods and mutant Bacillus thuringiensis strains are provided for controlling lepidopteran insects. Sporogenic, crystalliferous mutant strains for B. thuringiensis having the identifying characteristics of NRRL B-18195, NRRL B-18196 and NRRL B-18197 are provided for use as biocontrol agents. Said strains have the ability to produce a bypyramidal crystal composed of toxic protein and require a leucine and valine containing nutrient medium for growth, sporulation and crystal production.

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A. A. Yousten, "A Method for the Isolation of Asporogenic Mutants of Bacillus thuringiensis," Can. J. Microbiol. 24: 492-494, (1978).
K. W. Nickerson et al., "Physiology of Sporeforming Bacteria Associated with Insects: Minimal Nutritional Requirements for Growth, Sporulation, and Parasporal Crystal Formation of Bacillus thuringiensis," Appl. Microbiol., 28: 124-128, (1974).
S. M. Saleh et al., "Method for Determining Bacillus thuringiensis var. thuringiensis Berliner in Soil," Can. J. Microbiol., 15: 1101-1103, (1969).

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