Accumulation of heat shock proteins for evaluating biological da

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 29, 435 71, 435 731, 435 732, 435 722, 435 72, 436501, 436 8, 436 15, G01N 33567

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057802467

ABSTRACT:
Method of detecting chronic exposure of an organism to a stressor, and for evaluating biological damage due to chronic exposure to sublethal levels of stressors and kits for carrying out the method are disclosed. The methods comprise: (a) sampling at least one organism in order to determine whether it has been chronically exposed to a sublethal concentration of one or more stressors in its environment, under sampling conditions that do not induce any additional heat shock protein (hsp) response in the organism; (b) obtaining a sample of cells or secretions of said organism, suspected of having elevated levels of heat shock proteins and solubilizing the heat shock proteins in the sample; and (c) measuring the concentration of a heat shock protein in said sample. The invention also provides methods for evaluating biological damage due to chronic exposure to a sublethal concentration of one or more stressors comprising detecting chronic exposure of the organism by the above method and then comparing the measured concentration of hsp to a predetermined standard calibration curve which correlates hsp concentration with physiological impairment of growth or reproductive processes, and kits for carrying out the methods.

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