Accelerated nucleic acid reassociation method

Details Accelerated nucleic acid reassociation method Accelerated nucleic acid reassociation method

1991-01-23

1992-07-21

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 27, 935 1, 935 78, 935 80, C12Q 168, C07H 1512, C07H 1700

Patent

active

051322072

ABSTRACT:
A method for the formation of double stranded nucleic acid molecules from separate single stranded nucleic acid molecules in a single phase reaction solution is disclosed wherein the rate of reaction is greatly increased over the rate of reaction at standard reference conditions. The greatly accelerated reaction rate is accomplished through the use of known concentrations of nucleic acid precipitating agents which are added to the reaction solution. Nucleic acid denaturing agents may also be added. The solution so formed is incubated and then assayed for the presence of double stranded nucleic acid molecules.

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