Acarbose biosynthesis genes from actinoplanes sp., process for t

Details Acarbose biosynthesis genes from actinoplanes sp., process for t Acarbose biosynthesis genes from actinoplanes sp., process for t

1996-02-23

1998-05-19

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Vector, per se

536 2374, C12N 1563, C07H 2104

Patent

active

057535019

ABSTRACT:
The invention relates to acarbose biosynthesis genes from actinomycetes, predominantly from Actinoplanes sp. SE 50/110 and its mutants, to a process for the isolation of acarbose biosynthesis genes from actinomycetes using a gene probe which has been derived from highly conserved protein regions of known dTDP-glucose dehydratase enzymes for finding the genes acbA (coding for dTDP-glucose synthase), acbB (coding for dTDP-glucose dehydratase) and acbC (coding for a cyclase, in part identical to AroB, bacterial 3-dehydroquinate synthases) or one or more acarbose biosynthesis genes from Actinoplanes sp., and to the use of the acarbose biosynthesis genes.

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