Absorption-emission optrode and methods of use thereof

Chemistry: analytical and immunological testing – Nitrogen containing – Oxides of nitrogen

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2504591, 422 56, 422 57, 422 8206, 422 8207, 436 74, 436 79, 436121, 436172, G01N 2164, G01N 2165

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active

049295616

ABSTRACT:
A method and apparatus for monitoring the physical and chemical properties of a sample fluid by measuring an optical signal generated by a fluorescent substance and modulated by an absorber substance. The emission band of the fluorescent substance overlaps the absorption band of the absorber substance, and the degree of overlap is dependent on the physical and chemical properties of the sample fluid. The fluorescent substance and absorber substance are immobilized on a substrate so that an effective number of molecules thereof are sufficiently close for resonant energy transfer to occur, thereby providing highly efficient modulation of the fluorescent emissions of the fluorescent substance by the absorber substance.

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